LC-UV was performed using a Shimadzu LC-20A with 20AT binary pump and M20A PDA detector (Kyoto, Japan). Three different columns from Agilent were compared: the ZORBAX Eclipse plus C18 column, Eclipse plus C8 column and Eclipse XDB-CN column (250 × 4.6 mm, 5 μm). LC-HRMS was performed using a Thermo Fisher Vanquish Q-Exactive Plus system, which had a quaternary pump and an orbitrap mass analyzer (San Jose, CA, USA). An XP205 balance from Mettler Toledo (Greifensee, Switzerland) was used to weigh compounds.
M20a pda detector
Manufactured by Shimadzu
Sourced in Japan, United States
The M20A PDA detector is a lab equipment product manufactured by Shimadzu. It is a photodiode array (PDA) detector that is used for analytical applications. The M20A PDA detector provides high-speed, high-resolution detection for liquid chromatography (LC) and other analytical techniques.
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Lab products found in correlation
Eclipse plus c8 column, by Agilent Technologies (1 mentions)
Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)
Lc 20 hplc system, by Shimadzu (1 mentions)
Sil 20a ht autosampler, by Shimadzu (1 mentions)
Lcsolutions version 1.22 chromatography software, by Shimadzu (1 mentions)
Dgu 20a5 degasser, by Shimadzu (1 mentions)
2 protocols using m20a pda detector
1
Comparison of HPLC and HRMS Analytical Techniques
2
HPLC Analysis of Phenolic Compounds
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The HPLC analysis was conducted on a Shimadzu LC-20 HPLC system (LC-20AT quaternary pump, DGU-20A5 degasser, SIL-20A HT autosampler, and a SPD M20A PDA detector) running under Shimadzu LC Solutions version 1.22 chromatography software (Columbia, MD, USA). The column used was an Inertsil ODS-3 reversed-phase C18 column (5 µ, 250 mm × 4.6 mm from Varian, Palo Alto, CA, USA). For phenolic compound analysis, the initial conditions were 20% methanol and 80% water with 0.05 M phosphoric acid, at a flow rate of 1 mL/min. The effluent was monitored at 325 nm on the variable wavelength detector. After injection (typically 25 µL), the column was held at the initial conditions for 2 minutes and then developed to 100% methanol in a linear gradient over 55 minutes.
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