V9302 was synthesized and purified following a published paper[41 (link)]. 4-Vinylbenzyl chloride, 4,4-dithiodibutyric acid, triethylamine, 4-Cyano-4-(phenyl-carbonothioylthio)pentanoic acid, poly(Ethylene glycol)methyl ether methacrylate (average Mn = 950), 2,2-Azobis (isobutyronitrile)(AIBN), Dulbecco’s Modified Eagle’s Medium (DMEM), RPMI 1640 Medium, trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (MO, U.S.A). 2-deoxy-D-glucose was purchased from AK Scientific Inc. (CA, U.S.A.). Antibody against ASCT2 was purchased from Cell Signaling Technology, Inc. (MA, U.S.A). SuperSignal™ West Fento Maximum Sensitivity Substrate Kit and Pierce™ RIPA buffer were purchased from Thermo Scientific (MA, U.S.A). Fetal bovine serum (FBS), penicillin-streptomycin solution and Trizol lysis reagent were purchased from Invitrogen (NY, U.S.A.). QuantiTect Reverse Transcription Kit was purchased from Qiagen (MD, U.S.A). EnzyChrom Glutamine Assay kit was purchased from BioAssay Systems (CA, USA), Glucose Colorimetric/Fluorometric Assay Kit and Lactate Colorimetric/Fluorometric Assay Kit were purchased from BioVision Inc. (CA, USA). ALT/SGPT Liqui-UV Test Kit and AST/SGOT Liqui-UV Test Kit were purchased from Stanbio Laboratory (TX, USA).
Alt sgpt liqui uv test kit
Manufactured by EKF Diagnostics
Sourced in United States
The ALT/SGPT Liqui-UV Test kit is a diagnostic reagent used to measure the activity of the enzyme alanine aminotransferase (ALT) or serum glutamic-pyruvic transaminase (SGPT) in human serum or plasma. This test is commonly used to assess liver function.
Automatically generated - may contain errors
Lab products found in correlation
Lactate colorimetric fluorometric assay kit, by Abcam (1 mentions)
Glucose colorimetric fluorometric assay kit, by Abcam (1 mentions)
Trizol lysis reagent, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
4 vinylbenzyl chloride, by Merck Group (1 mentions)
4 4 dithiodibutyricacid, by Merck Group (1 mentions)
Enzychrom glutamine assay kit, by BioAssay Systems (1 mentions)
Pierce ripa buffer, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Quantitect reverse transcription kit, by Qiagen (1 mentions)
3 protocols using alt sgpt liqui uv test kit
1
Synthesis and Characterization of V9302 Compound
2
Inhibitory Effect of Berberine on GPT1
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To verify the direct inhibitory effect of BBR on GPT1, the enzymatic activity of purified recombinant human GPT1 protein (ab206804, Abcam, Cambridge, MA, USA), with or without BBR, was measured by an ALT/SGPT Liqui-UV Test kit (2930-500, Stanbio, Boerne, TX, USA) according to the manufacturer’s instructions. In detail, 500 mM L-Alanine, 15 mM α-KG, 1200 units/L lactate dehydrogenase, 0.18 mM NADH, and 0.01 mg/mL recombinant human GPT1 protein were combined with increasing concentrations of BBR (0, 1, 5, 10, 50, 100, 500, and 1000 μM) in a 200-μL reaction. The absorbance rate at 340 nm was recorded by a microplate reader (Labsystems, Vantaa, Finland) for about 5 min.
3
Quantitative ALT Assay in Cells and Tissue
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The ALT activity in both cells and tissue was measured by an ALT/SGPT Liqui-UV Test kit (2930-500, Stanbio, Boerne, TX, USA) according to the manufacturer’s instructions. In detail, cells (1 × 106) or tissue (50 mg) were rapidly lysed or homogenized with 200 μL ALT buffer (R1) and centrifuged at 12,000× g for 10 min at 4 °C. ALT working reagent was prepared in a ratio of 5 parts of buffer (R1) to 1 part of enzyme (R2). For each sample and blank control well of a 96-well UV plate, 200 μL working reagent was added and warmed to 37 °C. Then, 20 μL of cell or tissue homogenate was added to the sample well and gently mixed. The absorbance rate at 340 nm was recorded by a microplate reader (Labsystems, Vantaa, Finland) for about 5 min.
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