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Ammonia bicarbonate

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Ammonia bicarbonate is a chemical compound used in various laboratory applications. It is a white crystalline solid with the formula (NH4)HCO3. The compound serves as a pH buffer and can be used in chemical reactions and analytical procedures.

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Lab products found in correlation

Sequencing grade trypsin, by Promega (3 mentions) Urea, by Merck Group (3 mentions) Pngase f, by New England Biolabs (2 mentions) Triethylammonium acetate, by Merck Group (2 mentions) Sep pak c18 1cc vac cartridge, by Waters Corporation (1 mentions) 5 m nacl, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Sodium sulphite, by Merck Group (1 mentions) Tris 2 carboxyethyl phosphine, by Merck Group (1 mentions) Hydrazide beads, by Bio-Rad (1 mentions) Sodium periodate, by Bio-Rad (1 mentions) Acetonitrile, by Merck Group (1 mentions) Sep pak c18 1 cm3 vac cartridge, by Waters Corporation (1 mentions) Sep pak c18 resins, by Waters Corporation (1 mentions) Lys c, by Promega (1 mentions)

3 protocols using ammonia bicarbonate

1

Glycoprotein Enrichment and Identification

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Sodium periodate and hydrazide beads were purchased from Bio-Rad (Hercules, CA); bicinchoninic acid protein assay (BCA) assay kit was purchased from Pierce (Rockford, IL); sequencing-grade trypsin was from Promega (Madison, WI); Sep-Pak C18 1cc Vac Cartridge was from Waters (Milford, MA); PNGase F was from New England Biolabs (Ipswich, MA); Other chemicals such as urea, ammonia bicarbonate (AB), ACN, TFA, tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide, 5 M NaCl, sodium sulphite and phorbol 12-myristate 13-acetate (PMA) were purchased from Sigma-Aldrich (St. Louis, MO).
Yang W., Jackson B, & Zhang H. (2016). Identification of glycoproteins associated with HIV latently infected cells using quantitative glycoproteomics. Proteomics, 16(13), 1872-1880.
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2

Proteome Sample Preparation Protocol

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Sequencing-grade trypsin was from Promega (Madison, WI); Sep-Pak C18 1 cm3 Vac Cartridge was from Waters (Milford, MA); PNGase F was from New England Biolabs (Ipswich, MA). Other chemicals such as urea, ammonia bicarbonate (AB), acetonitrile (ACN), trifluoroacetic acid (TFA), tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide, triethylammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO).
Yang W., Shah P., Hu Y., Eshghi S.T., Sun S., Liu Y, & Zhang H. (2017). Comparison of Enrichment Methods for Intact N- and O-Linked Glycopeptides Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography. Analytical chemistry, 89(21), 11193-11197.
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3

Optimized Liquid Chromatography Mass Spectrometry

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C4 resin beads (35 μm, 300 Å) were purchased from Separation Methods Technologies (Newark, DE). Oasis MAX resins and Sep-Pak C18 resins were obtained from Waters (Milford, MA). Sequencing-grade trypsin and Lys-C were acquired from Promega (Madison, WI). Other chemicals including urea, ammonia bicarbonate (AB), acetonitrile (ACN), trifluoroacetic acid (TFA), triethyl ammonium bicarbonate (TEAB), tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide, and triethylammonium acetate were purchased from Sigma Aldrich (St. Louis, MO). Indexed retention time (iRT) standards (a mixture of eleven peptides) were purchased from Biognosys Inc (Zurich, Switzerland).
Dong M., Lih T.M., Chen S.Y., Cho K.C., Eguez R.V., Höti N., Zhou Y., Yang W., Mangold L., Chan D.W., Zhang Z., Sokoll L.J., Partin A, & Zhang H. (2020). Urinary glycoproteins associated with aggressive prostate cancer. Theranostics, 10(26), 11892-11907.
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