Lcms 8050 triple quadrupole liquid chromatography mass spectrometry system

Manufactured by Shimadzu

Sourced in Japan

The LCMS-8050 is a triple quadrupole liquid chromatography mass spectrometry (LC-MS/MS) system from Shimadzu. It is designed for the sensitive and accurate analysis of a wide range of compounds. The system combines a high-performance liquid chromatograph with a triple quadrupole mass spectrometer to provide precise quantitative and qualitative analysis.

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Lab products found in correlation

Labsolutions software, by Shimadzu (2 mentions) Xselect hss t3 column, by Waters Corporation (1 mentions)

Spelling variants of this product

LCMS-8050 (117 citations) Liquid chromatography system (32 citations) LCMS-8050 system (17 citations) Triple quadrupole LCMS-8050 (7 citations) Triple Quadrupole LCMS–8050 system (3 citations) Liquid chromatography/mass spectrometry (2 citations)

2 protocols using lcms 8050 triple quadrupole liquid chromatography mass spectrometry system

Measurement of Urinary Estrogens and Metabolites

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In urine, endogenous estrogens (estradiol and estrone) and their metabolites are present primarily in a conjugated form. Based on the criteria previously described, prior to sample analysis, a hydrolysis step is required to release endogenous estrogens and their metabolites in urine. Glucuronide and sulfate moieties were removed, allowing for the measurement of total levels of each estrogen and estrogen metabolite.
HPLC-MS/MS analysis was performed using a LCMS-8050 Triple Quadrupole Liquid Chromatography Mass Spectrometry system (Shimadzu, Kyoto, Japan) to measure estrogens and metabolites in urine samples (27 (link)). Quantification of estrogens and metabolites was carried out using LabSolutions software (Shimadzu). Molar quantities were standardized to creatinine accordingly.

Li X., Fang L., Li H, & Yang X. (2021). Urinary estrogen metabolites and breast cancer risk in Chinese population. Endocrine Connections, 10(12), 1615-1622.

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HPLC-MS/MS Metabolite Profiling

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HPLC mass spectrometry analysis was performed using a LCMS-8050 Triple Quadrupole Liquid Chromatography Mass Spectrometry system (Shimadzu). Both the HPLC and mass spectrometer were controlled by LabSolutions software (Shimadzu). HPLC was carried out on an XSelect HSS T3 column (3.5 μm, 2.1 × 100 mm, Waters) maintained at 45°C. A total of 10 μL of each sample was injected onto the column for separation. The mobile phase, operation at a flow rate of 0.3 mL/min, consisted of 0.2% trimethylamine (v/v) in water as solvent A and methanol as solvent B. A linear gradient, changing the A/B solvent ratio from 18:82 to 10:90 in 10 min, was employed. The general mass spectrometry conditions were as follows: ESI negative mode; spray voltage, -4 kV; sheath and auxiliary gas, nitrogen; scan type, multiple reaction monitoring (MRM) mode; collision gas, argon.

Zhang Q., You J., Zhu W., Wu Z, & Xiong J. (2020). Rapid Quantification of Urinary Organic Acids by HPLC Mass Spectrometry to Assess Nutritional Individuality in Chinese Children. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(10).

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