Genomic DNA from 158 pulverized, quick-frozen OC specimens was isolated using the DNeasy tissue-kit (Qiagen, Hilden, Germany). Targeted NGS was performed using the TruSight Cancer sequencing panel (Illumina, San Diego, USA). The analyses were performed on the Illumina MiSequ® and the NextSeq system (Illumina, CA, USA). After sequencing, mutations in BRCA1, BRCA2 and TP53 were identified with the help of NextGene and Geneticist Assistant softwares. Pathogenicity for new mutations that were not found in the database was determined and categorized using prediction tools like SIFT, alignGVGD, mutation taster.
Misequ
Manufactured by Illumina
Sourced in United States
The MiSequ is a next-generation sequencing (NGS) instrument developed by Illumina. It is designed to perform high-throughput DNA and RNA sequencing. The MiSequ utilizes Illumina's proprietary sequencing-by-synthesis technology to generate sequencing data. The core function of the MiSequ is to provide researchers with a robust and efficient platform for genomic analysis and applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using misequ
1
Targeted NGS of BRCA1/2 and TP53 in OC
2
Targeted NGS for Genomic DNA Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA from pulverized, quick-frozen OC specimens was isolated
using the DNeasy tissue-kit (Qiagen, Hilden, Germany). Targeted NGS was performed
using the TruSight Cancer sequencing panel (Illumina, San Diego, USA). The
analyses were performed on the Illumina MiSequ® and the NextSeq system (Illumina,
CA, USA). Mutation analysis was performed using NextGene and Geneticist Assistant
softwares.
using the DNeasy tissue-kit (Qiagen, Hilden, Germany). Targeted NGS was performed
using the TruSight Cancer sequencing panel (Illumina, San Diego, USA). The
analyses were performed on the Illumina MiSequ® and the NextSeq system (Illumina,
CA, USA). Mutation analysis was performed using NextGene and Geneticist Assistant
softwares.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!