Rpmi 1640 c11875500bt

Human PDAC cell lines (BxPC-3, PANC-1, and SW-1990) and the human embryonic kidney cell line, HEK 293, were obtained from the Type Culture Collection Committee of the Chinese Academy of Sciences (Shanghai, China). The Human PDAC cell line, MIA PaCa-2, was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). Mycoplasma contamination was excluded in all cell lines. The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; C11995500BT, Gibco, Grand Island, CA, USA) or RPMI-1640 (C11875500BT; Gibco, Grand Island, CA, USA) basic medium with 10% fetal bovine serum (FBS; 10099141C, Gibco, Grand Island, CA, USA) at 37°C in a humidified at 5% CO₂.

Taxus wallichiana var. chinensis (Pilg.) Florin was purchased from the pharmacy in the First Affiliated Hospital of Zhejiang Chinese Medical University (Hangzhou, China), which was manufactured by the Ningbo Taikang Yew Biological Engineering Company (Batch No. 100513). Human lung cancer (LC) cell lines, including A549, NCI-H358, HCC827, and MRC-5, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). They were cultured by 0.25% trypsin digestion in the mediums of F-12K (21127-022, GIBCO, Hangzhou, China), RPMI-1640 (C11875500BT, GIBCO, Hangzhou, China), and MEM (41500034, GIBCO, Hangzhou, China) for 1–2 mins at 37°C in a humidified atmosphere consisting of 5% CO₂ separately, where NCI-H358 and HCC827 were both cultured in RPMI-1640 medium. The antibodies included N-cadherin (1:3000, ab98952, Abcam), E-cadherin (1:2000, 20874-1-AP, Proteintech), vimentin (1:2000, ab92547, Abcam), p-JAK (1:500, ab32101, Abcam), p-STAT3 (1:500, ab76315, Abcam), JAK (1:1000, 3230, CST), STAT3 (1:1000, 06-596, Merck), and GAPDH (1:5000, 70-Mab5465-040, MultiSciences).