In the experiment, 0.05 mol/L phosphate buffered saline (PBS) of pH 8.0 was prepared first, and then the following solutions were prepared: 1.5 mmol/L Acetylthiocholine iodide (ATChI); 0.75 mmol/L 5, 5'-Dithiobis-(2-nitrobenzoic acid) (DTNB, Sigma Aldrich, St. Louis, MO); 0.2 U/mL Acetylcholinesterase (AChE, from electric squid, Macklin); 0.5 U/mL Butyrylcholinesterase (BuChE, from equine serum, Aladdin, Shanghai, China); 1.5 mmol/L S-Butyrylthiocholine iodide (BTChI, Sigma Aldrich, St. Louis, MO). 4% sodium dodecyl sulphate (SDS) was prepared with secondary water. The test compounds and the positive control Donepezil (Sigma Aldrich, St. Louis, MO) were formulated with DMSO to a gradient of 0.1, 1.0, 10.0, 50.0, 100.0, 500.0, and 1000.0 μg/mL. The above reagents were all analytically pure, and the test water was all distilled water. All solutions were stored in a refrigerator at 4 °C.
S butyrylthiocholine iodide btchi
Manufactured by Merck Group
Sourced in China
S-Butyrylthiocholine iodide (BTChI) is a chemical compound that is commonly used in biochemical and analytical applications. It functions as a substrate for the enzyme butyrylcholinesterase, which catalyzes its hydrolysis. The product of this reaction can be detected and measured, making BTChI a useful tool for various laboratory analyses.
Automatically generated - may contain errors
Lab products found in correlation
5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (2 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Donepezil, by Merck Group (1 mentions)
Donepezil hydrochloride, by Merck Group (1 mentions)
Butyrylcholinesterase bche, by Merck Group (1 mentions)
Acetylthiocholine iodide atchi, by Merck Group (1 mentions)
Electrophorus electricus ache, by Merck Group (1 mentions)
Recombinant human ache, by Merck Group (1 mentions)
2 protocols using s butyrylthiocholine iodide btchi
1
Cholinesterase Inhibition Assay Protocol
2
Cholinesterase Enzyme Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cholinesterase enzymes viz. Electrophorus electricus AChE (EC 3.1.1.7, from electric
eel, 1256 U/mg of protein), recombinant human AChE (EC 3.1.1.7, recombinant,
expressed in HEK 293 cells, ≥1000 units/mg protein), and butyrylcholinesterase
(BChE, E.C. 3.1.1.8, from equine serum, 855 U/mg protein) were purchased
from Sigma-Aldrich. The reagents required in the assay viz. 5,5-dithiobis-(2-nitrobenzoic
acid) (DTNB), acetylthiocholine iodide (ATChI), S-butyrylthiocholine iodide (BTChI), and reference standard donepezil
hydrochloride were also purchased from Sigma-Aldrich.
eel, 1256 U/mg of protein), recombinant human AChE (EC 3.1.1.7, recombinant,
expressed in HEK 293 cells, ≥1000 units/mg protein), and butyrylcholinesterase
(BChE, E.C. 3.1.1.8, from equine serum, 855 U/mg protein) were purchased
from Sigma-Aldrich. The reagents required in the assay viz. 5,5-dithiobis-(2-nitrobenzoic
acid) (DTNB), acetylthiocholine iodide (ATChI), S-butyrylthiocholine iodide (BTChI), and reference standard donepezil
hydrochloride were also purchased from Sigma-Aldrich.
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