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Anti trim54

Manufactured by Proteintech
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Anti-TRIM54 is an affinity purified polyclonal antibody raised in rabbit against a recombinant protein fragment corresponding to a region within amino acids 1 - 200 of human TRIM54. TRIM54 is a tripartite motif-containing protein involved in muscle development and function.

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Lab products found in correlation

Anti flag, by Merck Group (1 mentions) Anti β catenin, by Abcam (1 mentions) Anti myc, by Proteintech (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti ha, by Abcam (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Z vad fmk, by Merck Group (1 mentions) Necrosulfonamide, by Merck Group (1 mentions) Anti slc7a11, by ABclonal (1 mentions) Anti tfrc, by ABclonal (1 mentions) Ferrostatin 1, by Targetmol (1 mentions) Anti gpx4, by Abcam (1 mentions) Pd98059, by MedChemExpress (1 mentions) Sorafenib, by Targetmol (1 mentions)

Close spelling variants of this product

Anti-TM (2 citations)

2 protocols using anti trim54

1

Western Blotting Protein Detection

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Western blotting was performed according to the manufacturer’s instruction, using the primary antibodies, anti-flag (Sigma, F3165), anti-TRIM54 (Proteintech, 21074-1-AP), anti-myc (Proteintech, 16286-1-AP), anti-Axin1 (Proteintech, 16541-1-AP), anti-HA (Abcam, ab9110), and anti-β-catenin (Abcam, ab32572). Following the initial Western blot assay, the membranes were stripped and re-probed with anti-α-tubulin or anti-p84 (Sigma, Saint Louis, MO, USA) as a protein loading control.
Zhu J., Wu Y., Lao S., Shen J., Yu Y., Fang C., Zhang N., Li Y, & Zhang R. (2021). Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma. Frontiers in Oncology, 11, 759842.
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2

Antibody-Based Ferrostatin-1 Pathway Study

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Antibodies used in this study include anti-GAPDH (60004-1-Ig, Proteintech), anti-FSP1 (20886-1-AP, Proteintech), anti-GPX4 (ab125066, Abcam), anti-TFRC (A5865, ABclonal), anti-SLC7A11 (A2413, ABclonal), anti-TRIM54 (21074-1-AP, Proteintech), anti-ERK (#4695, Cell Signaling Technology), anti-ubiquitin (sc-9133, Santa Cruz Biotechnology), and horseradish peroxidase-conjugated secondary antibodies (VA001 and VA002, Vicmed). Reagents used in this study include Sorafenib (T0093L, Targetmol), Ferrostatin-1 (A4371, APE × BIO), Z-VAD-FMK (A1902, APE × BIO), Necrosulfonamide (B7731, APE × BIO), cycloheximide (C7698-1G, SIGMA), MG-132 (A2585, APE × BIO), and PD98059 (HY-12028, MedChemExpress).
Liu M.R., Shi C., Song Q.Y., Kang M.J., Jiang X., Liu H, & Pei D.S. (2023). Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1 ubiquitination and degradation in hepatocellular carcinoma. Hepatology Communications, 7(10), e0246.
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