Salivary cortisol levels were determined with a high-sensitivity salivary cortisol competitive immunoassay kit (Salimetrics, State College, PA, United States) according to the manufacturer's instructions. All samples were analyzed in duplicate. After assay completion, optical density was read on a BioTek ELx808 microplate reader at 450 nm. Cortisol concentration was calculated using Gen 5 v. 3.08 (BioTek Instruments, Winooski, VT, United States) with a 4-parameter non-linear regression standard curve. Assay quality was measured by calculating the intra-assay coefficient of variation (CV) and inter-assay variability, which were 2.63% and 3.38%, respectively. Samples with higher values than the highest standard were diluted and re-analyzed. The upper limit of quantification was 82.77 nmol/l, and the lower limit was 0.19 nmol/l.
High sensitivity salivary cortisol competitive immunoassay kit
Manufactured by Salimetrics
Sourced in United States, Germany
The High-sensitivity salivary cortisol competitive immunoassay kit is a laboratory equipment product manufactured by Salimetrics. The kit is designed to measure cortisol levels in saliva samples using a competitive immunoassay technique. The core function of this product is to quantify salivary cortisol concentrations.
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2 protocols using high sensitivity salivary cortisol competitive immunoassay kit
1
Salivary Cortisol Quantification Protocol
2
Salivary Cortisol Measurement Protocol
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Cortisol was determined from saliva collected using a Salivette® device (Sarstedt, Nümbrecht, Germany). Saliva was extracted from the Salivette by centrifugation (2500 rpm, 5 min) and frozen at -20°C until assay. Salivary cortisol levels were assayed by an independent laboratory (Daacro's Saliva Lab Trier, Germany) using a high sensitivity salivary cortisol competitive immunoassay kit (Salimetrics, LLC, U.S.A.). After assay completion, optical density was read on a BioTeK ELx808 microplate reader at 450 nm (with correction at 490 nm). The intra-and inter-assay CVs were 3.54% and 4.39% respectively.
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