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Murine colon carcinoma cell line MC38 was ordered from Suzhou LongMab Biosciences, Suzhou, China. BMDCs were separated from female C57BL/6 mice and sorted using EasySep™ Mouse CD11c Positive Selection Kit II (STEMCELL, Vancouver, Canada) according to the manufacturer's instructions. Anti-mouse CD16/32 antibody and mouse antibodies of FITC-H-2Kb/H-2Db, FITC-I-A/I-E, PE/APC-CD11c, FITC-CD40, FITC-CD80, FITC-CD86 and PE-CCR7 were all purchased from BioLegend, San Diego, CA, USA. All cytokines used in this work were kindly provided by Novoprotein, Shanghai, China. Primary anti-NY-ESO-1 and anti-His rabbit antibodies are CST products (Danvers, MA, USA), and secondary goat anti-rabbit Alexa fluor 488 antibody is a product of Invitrogen, Carlsbad, CA, USA. The proteasome inhibitor lactacystin was bought from APExBIO, Houston, TX, USA. SYBR GREEN qPCR mix was purchased from Vazyme Biotech, Nanjing, China. All ELISA kits and the mouse IFN-γ ELISPOT kit were obtained from DAKEWE Biotech, Beijing, China. Mouse MIP-3β (CCL19) was purchased from Peprotech, Cranbury, NJ, USA.