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Pregnant female rats

Manufactured by Charles River Laboratories

Pregnant female rats are laboratory animals used for scientific research. They are commonly utilized to study the effects of various factors on fetal development and growth.

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2 protocols using pregnant female rats

1

Pregnant Rat Housing and Care

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Six time-mated, pregnant female rats were purchased from Charles River (Germany). After birth of the litter they were housed one litter per cage with free access to food and water under a 12 hours light/12 hours dark cycle in a temperature-controlled room. All experimental procedures performed in this study were approved by the Ethical Committee for Use of Laboratory Animals in the Lund-Malmö region.
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2

Microglia and Sprague-Dawley Rat Models

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Adult Sprague–Dawley rats (40- to 50-d-old; 8 males and 15 females) from Charles River Laboratories were used in generating tissue homogenates for Western blot, ELISA and Chemotaxis Assay. Ten pregnant female rats (Charles River) produced neonatal pups for primary microglia culture. All experimental protocols and animal care were carried out in accordance with the National Institutes of Health Guide for the Care of Laboratory Animals in Research and approved by the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center, and by Shanghai Children’s Medical Center as well. All efforts were made to minimize animal suffering and the number of animals used in this study.
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