Flot 1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Flot-1 is a lab equipment product offered by Santa Cruz Biotechnology. It is designed for use in research applications. The core function of Flot-1 is to facilitate the isolation and analysis of lipid rafts from cell membranes.

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Lab products found in correlation

2 protocols using flot 1

Glycolytic Enzyme Expression Analysis

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Control or BME treated cell lysates were prepared, and western blot analysis was performed using specific antibodies to GLUT-1, PFKP, LDHA, PDK3, ACLY, FASN, and Flot-1 (Santa Cruz Biotechnology), ACC1 and CHOP (Cell Signaling Technology). Anti-mouse or anti-rabbit secondary antibodies were purchased from BIO-RAD. The blot was reprobed with actin-HRP antibody to compare protein load in each lane. Densitometry analysis was done using Image J software (NIH).

Sur S., Nakanishi H., Flaveny C., Ippolito J.E., McHowat J., Ford D.A, & Ray R.B. (2019). Inhibition of the key metabolic pathways, glycolysis and lipogenesis, of oral cancer by bitter melon extract. Cell Communication and Signaling : CCS, 17, 131.

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Cell Line Maintenance and Antibody Validation

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The SCLC cell lines NCI-H446 and NCI-H1688 were purchased from the Cellular Biology Institute of the Shanghai Academy of Sciences (Shanghai, China). Both cell lines were maintained in RPMI-1640 medium supplemented with 100 U/mL penicillin, 100 μg/mL phytomycin, and 10% fetal calf serum (FCS), and were cultured in a humidified atmosphere in an incubator at 37°C and with 5% CO₂. RPMI-1640, PBS RPMI-1640, and FCS were purchased from Gibco-BRL (Life Technologies, Paisley, Scotland). Antibodies against total AKT, p-AKT, smad2/3, and p-smad2/3 were all obtained from Cell Signaling Technology, Inc. (CST, CA, USA). Antibodies against E-cadherin, cyclinD1, vimentin, caspase-3, PARP, TGF-β, MMP9, and Flot1 were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Zhao L., Li J., Liu Y., Zhou W., Shan Y., Fan X., Zhou X., Shan B., Song Y, & Zhan Q. (2018). Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway. Cancer Biology & Medicine, 15(4), 400-414.

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