W plan apo 1.0 na 20x water immersion objective

Manufactured by Zeiss

The W-Plan-Apo/1.0 NA 20x water immersion objective is a high-numerical aperture objective lens designed for microscopy applications. It has a magnification of 20x and a numerical aperture of 1.0, providing high-resolution imaging capabilities. This objective is suitable for use with water-based samples.

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Lab products found in correlation

Spectra x light engine led light, by Lumencor (3 mentions) Axio examiner z1, by Zeiss (3 mentions) Plan apochromat 20x 0.8 na air objective, by Zeiss (2 mentions) Orca flash 4.0 scmos camera, by Hamamatsu Photonics (2 mentions) Orca flash 4, by Hamamatsu Photonics (1 mentions) Axio observer z1, by Zeiss (1 mentions)

Close spelling variants of this product

20X-W/1.0 NA objective Plan Apo NA 0.80 Zeiss 20x (1.0 NA) 20× water immersion objective Plan Apo objective

3 protocols using w plan apo 1.0 na 20x water immersion objective

Spontaneous Activity Imaging in Neuroscience

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Spontaneous activity imaging was performed on an upright AxioExaminer Z-1 (Zeiss), equipped with a Spectra-X light engine LED light (Lumencor), and controlled with Slidebook software (Intelligent Imaging Innovations). Images were acquired using a W-Plan-Apo/1.0 NA 20x water immersion objective (Zeiss). Images (2,048 × 400 px², pixel size: 0.325 × 0.325 μm²) were collected continuously on an OrcaFlash4.0 sCMOS camera (sCMOS; Hamamatsu) at a sampling rate of 0.5 kHz, with 4 × 4 binning, and a 633 nm LED excitation light power of 6.5 mW/mm². 6 × 10 s movies were recorded sequentially for each imaging area.

Walker A.S., Raliski B.K., Nguyen D.V., Zhang P., Sanders K., Karbasi K, & Miller E.W. (2021). Imaging Voltage in Complete Neuronal Networks Within Patterned Microislands Reveals Preferential Wiring of Excitatory Hippocampal Neurons. Frontiers in Neuroscience, 15, 643868.

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Spontaneous Activity Imaging of Neurons

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Spontaneous activity imaging was performed on an upright AxioExaminer Z-1 (Zeiss) or an inverted Zeiss AxioObserver Z-1 (Zeiss), both equipped with a Spectra-X light engine LED light (Lumencor), and controlled with µManager (V1.4, open-source, Open Imaging) (Edelstein et al., 2014) . Images were acquired using a W-Plan-Apo/1.0 NA 20x water immersion objective (Zeiss) or a Plan-Apochromat/0.8 NA 20x air objective (Zeiss).
Images (2048 x 400 px 2 , pixel size: 0.325 x 0.325 μm 2 ) were collected continuously on an OrcaFlash4.0 sCMOS camera (sCMOS; Hamamatsu) at a sampling rate of 0.5 kHz, with 4x4 binning, and a 633 nm LED excitation light power of 6.5 mW/mm 2 . 6 x 10 s movies were recorded sequentially for each imaging area.

Walker A., Raliski B.K., Nguyen D.V., Zhang P., Sanders K., Karbasi K., & Miller E.W. (2020). Imaging voltage in complete neuronal networks within patterned microislands reveals preferential wiring of excitatory hippocampal neurons.

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Simultaneous Calcium Imaging in 3D

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Spontaneous activity imaging was performed on an upright AxioExaminer Z-1 (Zeiss) or an inverted Zeiss AxioObserver Z-1 (Zeiss), both equipped with a Spectra-X light engine LED light (Lumencor), and controlled with Slidebook (3i). Images were acquired using a W-Plan-Apo/1.0 NA 20x water immersion objective (Zeiss) or a Plan-Apochromat/0.8 NA 20x air objective (Zeiss). Images (2048 x 400 px 2 , pixel size: 0.325 x 0.325 μm 2 ) were collected continuously on an OrcaFlash4.0 sCMOS camera (sCMOS; Hamamatsu) at a sampling rate of 0.5 kHz, with 4x4 binning, and a 633 nm LED excitation light power of 13 mW/mm 2 .

Walker A., Raliski B.K., Karbasi K., Zhang P., Sanders K., & Miller E.W. (2020). Optical spike detection and connectivity analysis with a far-red voltage-sensitive fluorophore reveals changes to network connectivity in development and disease.

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