Um200 oven

To evaluate the effect of B toxicity on plant growth and morphology, leaf area, together with leaf, stem, and root dry masses were recorded. The roots were assessed following removal of the substrate via gentle washing. Leaf area (one-sided surface area) was determined with a leaf area meter (Li-3000A; Li-Cor Inc., Lincoln, NE, USA), and dry weight (DW) was assessed (±0.01 g; MXX-412; Denver Instruments, Bohemia, NY, USA) after drying the tissue at 75 • C for 48 h (UM200 oven, Memmert GmbH, Schwabach, Germany). Root-to-shoot ratio (root mass/shoot mass), specific leaf area (SLA; leaf area/leaf mass), and leaf mass ratio (LMR; leaf mass/plant mass) were calculated.
Five and ten days following destructive measurements (i.e., 40 and 45 d of growth, respectively), leaf images were obtained in another set of plants by using a digital camera (Canon EOS M2; Canon Inc., Tokyo, Japan). The camera-to-leaf distance was maintained constant.

Small woody fruits containing thousands of tiny seeds of C. citrinus and C. linearis were collected directly from a total of 5 shrubs per species and stored in paper bags on November 2019. The unopened fruits should be incubated (Memmert UM200 oven) in a paper bag at 40 °C for 48 hours until a great number of fine seeds were released (Figure 1). For each species, the seed sample was obtained by mixing the seeds to minimize inter-genetic variation (Kheloufi et al. 2018) (link).
Seeds were selected properly, described, photographed (using a Coolpix Nikon P610, 16 megapixels) and then stored in a bottle glass at 4°C for one month. The length and the width of 50 seeds for each species were measured using Digimizer Image Analysis Software (version 5.4.3, MedCalc, Ostend, Belgium) . A scale bar was added.
After processing, seeds were reserved for germination test. Seed provenances and morphological characteristics are shown on Table 1.