Elecsys progesterone 2 kits

Blood from the tail vein was sampled into evacuated heparinized blood tubes (Vacutainer, Becton Dickinson, Auckland, New Zealand) on Days 0, 7, and 15 of the embryo flush cycles for progesterone concentration measurement. Blood samples were immediately placed in ice water, centrifuged at 4°C for 15 min at 1,500 × g, and aspirated plasma was stored at -20°C until assayed.
Progesterone concentrations were measured by New Zealand Veterinary Pathology Ltd. (Hamilton, New Zealand) with an electro chemiluminescence detection system (Elecsys Progesterone II kits, Roche Diagnostics, GmbH, Germany) using an automated clinical immunology analyzer (Roche Modular E170, Basel, Switzerland) . The sensitivity of the progesterone assay was 0.03 ng•mL -1 , with a coefficient of variation of less than 5%. Samples measured below the sensitivity of the assay were assigned the value of the sensitivity of the assay (2% of measurements made on Day 0).

Blood was sampled from the tail vein into evacuated heparinized blood tubes (Vacutainer; Becton Dickinson, Auckland, New Zealand) on Days 2, 5, 7, and 15 of the embryo flush cycles for progesterone measurement. Blood samples were placed immediately in ice water, and then centrifuged at 4°C for 15 min at 1,500 × g. Aspirated plasma was stored at -20°C until assayed.
Progesterone concentrations were measured with an electro chemiluminescence detection system (Elecsys Progesterone II kits, Roche Diagnostics GmbH, Mannheim, Germany) using an automated clinical immunology analyzer (Roche Modular E170, Basel, Switzerland) at New Zealand Veterinary Pathology Ltd. (Hamilton, New Zealand) . The sensitivity of the assay was 0.03 ng•mL -1 and the co-efficient of variation was <5%. Only one sample had a concentration <0.03 ng•mL -1 , and this measurement was assigned the value of the sensitivity of the assay (0.03 ng•mL -1 ).