Expression of uorescent marker genes (i.e., ZsGreen, DsRed, and tdTomato) in plant tissues was observed using a Stemi SVII stereomicroscope equipped with an HBO illuminator (Zeiss, Thornwood, NY, USA), a FITC lter set (λ excitation = 480 nm, and λ emission = 515 nm; Chroma Technology, Bellows Falls, VT, USA), and a DsRED lter (λ excitation = 545/25 nm, dichroic 565LP, λ emission = 605/70 nm; Chroma Technology). Expression of ZsGreen in isolated embryos was observed under a Zeiss Axioskop 2 plus uorescence microscope equipped with an 89 North ® PhotoFluor LM-75 illumination system (Chroma Technology) and a FITC lter set. Images were taken using an AxioCam camera (Carl Zeiss, Oberkochen,
Germany) and the AxioVision LE64 software.
Zhang Z., Guo Y., Marasigan K., Conner J.A., & Ozias‐Akins P. (2021). Gene Activation via Cre/lox-mediated Excision in Cowpea (Vigna unguiculata).
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