Xe 2100 fully automatic hematology analyzer

We collected 5 ml of peripheral venous blood from the patient before the first cycle of treatment (baseline), before the second and third cycles of treatment, and at the time of progression detection, centrifuged at 3000 rpm for 10 minutes, and quickly stored the supernatant in a -20 °C refrigerator for reserve. As is standard, VEGF-A detection was performed using a Weigao JR-1 Chemiluminescent Immunoassay Analyzer and Vascular Endothelial Growth Factor Assay Kit (chemiluminescence) (Shandong Weigao Group Medical Polymer Co., Ltd., Weihai, China) according to the manufacturer's instructions, and the technicians did not have access to the clinical data. A complete blood count was performed using a Sysmex XE-2100 fully automatic hematology analyzer with original matching reagents, calibration and quality control products (Sysmex Corporation, Kobe, Japan), and the SII was calculated using the following formula: SII = platelet count ×neutrophil count/lymphocyte count.

Multidimensional assessments, including sociodemographic characteristics, asthma duration, smoking history, allergen detection results, comorbidities, medication use and acute asthma exacerbation, were performed for all included patients. Asthma control was assessed using the Asthma Control Questionnaire (ACQ) (12 (link)), the Mini Asthma Quality of Life Questionnaire (Mini-AQLQ) (13 (link)), and the Asthma Control Test (ACT) (14 (link)).
The participants also underwent spirometry, FeNO tests, routine blood tests and serum total IgE detection. The FeNO test was performed using a FeNO analyzer (NIOX MINO, Aerocrine AB, Sweden). After the FeNO test, pulmonary function and bronchial dilation tests were performed using spirometry (Master Screen-PFT, Jaeger, Germany). Routine blood tests were performed with a hematology analyzer (Sysmex XE-2100 Fully Automatic Hematology Analyzer, Sysmex, Japan). Serum total IgE was measured by immunoassay (Hitachi 7600 automated biochemistry analyzer, Hitachi, Japan), with a minimum detectable level of IgE of 1.0 IU/mL.