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44 protocols using avatar 370

1

Characterization of PEGDA-AESO Copolymers

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Fourier-transform infrared spectroscopy (FTIR) was used to confirm the formation of PEGDA:AESO copolymers at different ratios and to monitor the conversion of the acrylic double bonds. The infrared analysis was performed using a Thermo Avatar 370 spectrophotometer equipped with an attenuated total reflectance (ATR) device with a diamond crystal for solid analysis. Using Omnic 7.3 software, the spectra were collected in absorbance mode with a resolution of 4 cm−1 and 32 scans per measurement, within the range of 2000 to 650 cm−1, using 3D-printed dog-bone specimens. The conversion of double bonds was calculated from the decrease in the area of the double-bond absorption peak at 810 cm−1, as shown by Equation (2).
Conversion %=1At810A0810×100%,
where A0(810) and At(810) are the areas of the peak at 810 cm−1, before and after exposure to UV irradiation, respectively.
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2

Characterization of Adsorbent Samples

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The instruments used to characterize the structures of the samples as well as to determine adsorbate concentrations are listed in Table 6.

Instruments and methods.

Characterization method/instrumentModel/companyCountry
Fourier transform infrared (FT-IR)Avatar 370, Thermo NicoleUSA
Field emission scanning electron microscopy-energy dispersive X-ray (FESEM-EDX)SIGMA HV, ZeissGermany
N2 adsorption–desorption isotherm measurements (porosimetry analyzer)Belsorp-mini II, BELJapan
Powder X-ray diffraction (PXRD)AW-XDM300, AsenwareChina
Flame atomic absorption spectroscopy (FAAS)PerkinElmer Model A300Norwalk, USA
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3

Comprehensive Characterization of Hydroxyapatite

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HAP was characterized using optical microscopy, SEM, and FT-IR. Electron microscopy was completed using a Zeiss EVO LS10 microscope operated at 2.0 kV. FT-IR spectra were combined from 64 scans on a Thermo Nicolet Avatar 370 with a diamond crystal Smart iTR attachment. QCM crystals were prepared as above. Surface roughness of spin-coated HAP was determined by AFM (Bruker Nanoscope III multimode, Santa Barbara, CA) in contact mode with a SiN4 cantilever. Surface area measurements were obtained using the instrument’s accompanying analysis software.
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4

Nanoparticle Characterization Techniques

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Morphology and size distribution of nanoparticles were evaluated using transmission
electron microscope (TEM) (Gatan model 791, Philips CM 12, Poland) and image J,
respectively. X-ray Diffraction (XRD) patterns of dried nanoparticle powders were analyzed
through the GNR EXPLORER (XRD) X-ray diffraction gauge with a wavelength of 1.54 Å
Ka lines. Fourier Transform Infrared (FTIR) spectra were evaluated using an
infrared spectrometer Red (AVATAR 370, Thermo Nicolet AVATAR 370 FTIR, USA). The surface
charge of nanoparticles and the hydrodynamic size of nanoparticles were measured by a
device (SZ100, HORIBA, Japan). The contents of bismuth in the samples were measured using
Inductively Coupled Plasma - Optical Emission Spectrometry (ICP-OES) (VISTA-MPX, Varian
Inc., USA).
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5

Characterization of Aluminum Disks by FTIR

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Both uncoated and DURALTI®-coated aluminum disks were characterized by Fourier transform infrared spectroscopy (FTIR). FTIR spectra were recorded with a Thermo Avatar 370, equipped with an attenuated total reflection (ATR) device using a diamond crystal as an internal reflectance element. Spectra were acquired at room temperature in the range from 4000 to 530 cm−1, with 32 scans and a resolution of 4 cm−1.
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6

Synthesis and Characterization of 125I-Labeled Nanographene Oxide

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Carrier-free Na125I was purchased from PerkinElmer (Waltham, MA, USA), chloramine-T was sourced from Merck Chemicals Co., Ltd. (Shanghai, People’s Republic of China), and the other chemicals were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). The synthesis and characterization of NGO and 125I-NGO was performed as previously described.24 NGO was synthesized from natural graphite by oxidation with H2SO4/KMnO4 according to a modified Hummers method. The NGO was characterized by Fourier transform infrared spectroscopy (Avatar 370; Thermo Fisher Scientific Inc., Waltham, MA, USA), Raman spectroscopy (LabRam-1B; JY Co., Ltd., Dilor, France), scanning electron microscopy (1530VP; LEO Corporation, Oberkochen, Germany), transmission electron microscopy (JEOL 2010; JEOL Ltd., Tokyo, Japan), and atomic force microscopy (Nanoscope III; Veeco Metrology Group, Santa Barbara, CA, USA). 125I-NGO was synthesized according to the chloramine-T (N-chloro-p-toluenesulfonic acid) method. The radiochemical purity and stability of 125I-NGO was then examined.
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7

FTIR Spectroscopic Analysis of Films

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The infrared spectrum of absorption or emission of a matter was determined using Fourier transform infrared spectroscopy (FTIR) (Thermo Nicolet, Avatar 370). At first, the films were powdered to set up the discs, and then 70 mg of spectroscopic grade KBr was mixed fully with roughly 2 mg of the film's powder. Subsequently, the powder was hard‐pressed into pellets to obtain a transparent disc with 15 mm in diameter and 0.54 mm thickness. The FTIR spectra were obtained in the 4000–400 cm−1 range, at 25°C, by co‐adding 32 scans with 4 cm−1 spectral resolution.
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8

FTIR Characterization of Drug-Polymer Interactions

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The IR spectra were recorded on Thermo Nicolet, Avatar 370. FTIR spectra of sodium alginate, HPMC and the drug (Ciprofloxacin HCl) were obtained. Spectral scanning was done in the range between 4000 and 500 cm−1[6] , [17] . FT-IR study of drug and polymer mixture along with water (in situ mixture) is shown in Fig. 6.
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9

Chemical Characterization of Polysaccharides by FTIR

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Fourier transform infrared spectroscopy (FTIR; Thermo Nicolet Avatar 370, San Diego, CA, United States) was used to study the chemical structures of CMC, SCMC, к-carrageenan, and CM-к-Car.
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10

Polysaccharide Structural Characterization

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Fourier Transform Infrared spectrum for the polysaccharide sample was acquired in transmittance mode with Thermo Nicolet, Avatar 370 spectrometer to analyze different functional groups. Compressed discs of 3 mm diameter were prepared by mixing with 2 mg of lyophilized EPS with 200 mg of KBr, and the spectrum was corrected for KBr background. The pellets were then scanned in the range of 4000–500 cm−1 with a resolution 4 cm−1 and using 32 scans.
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