Human pathogenic bacteria of representative groups such as Gram positive bacteria (Staphylococcus aureus MTCC 96, Micrococcus luteus MTCC 1538, and Bacillus subtilis MTCC 441), Gram negative bacteria (Escherichia coli MTCC 443, Proteus vulgaris MTCC 426, and Shigella flexneri MTCC 1457), and fungi (Candida albicans MTCC 227 and Trichoderma viride MTCC 800) were obtained from Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Chandigarh for the antimicrobial studies. Bacteria were sub-cultured on nutrient broth medium (g/L: peptone 10.0, beef extract 10.0, sodium chloride 5.0, and pH 7.2), while fungi were grown on potato dextrose broth medium (g/L; potatoes, infusion from 200.0, dextrose 20, and pH 5.2) for further experimental purposes.
Shigella flexneri
Manufactured by Microbial Type Culture Collection
Sourced in India
Shigella flexneri is a Gram-negative bacterium that belongs to the Shigella genus. It is a well-known causative agent of bacillary dysentery, a severe form of gastroenteritis. This bacterial culture is commonly used in research and laboratory settings to study the pathogenesis and epidemiology of shigellosis.
Automatically generated - may contain errors
Lab products found in correlation
Staphylococcus aureus, by Microbial Type Culture Collection (2 mentions)
Salmonella typhi, by Microbial Type Culture Collection (2 mentions)
Bacillus subtilis, by Microbial Type Culture Collection (2 mentions)
Bacillus cereus, by Microbial Type Culture Collection (2 mentions)
Staphylococcus aureus mtcc 96, by Microbial Type Culture Collection (1 mentions)
Bacillus subtilis mtcc 441, by Microbial Type Culture Collection (1 mentions)
Escherichia coli mtcc 443, by Microbial Type Culture Collection (1 mentions)
Escherichia coli, by Microbial Type Culture Collection (1 mentions)
Candida albicans, by Microbial Type Culture Collection (1 mentions)
Staphylococcus epidermidis, by Microbial Type Culture Collection (1 mentions)
Vibrio parahaemolyticus, by Microbial Type Culture Collection (1 mentions)
Klebsiella pneumonia, by Microbial Type Culture Collection (1 mentions)
Listeria monocytogenes, by Microbial Type Culture Collection (1 mentions)
4 protocols using shigella flexneri
1
Antimicrobial Evaluation of Representative Microbes
2
Microbial Pathogen Culture Collection and Characterization
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All the test microbial pathogens such as Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 121), Bacillus cereus (MTCC 430), Staphylococcus epidermidis (MTCC 435), Escherichia coli (MTCC 7410), Klebsiella pneumonia (MTCC 7407), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio parahaemolyticus ((MTCC 451), Xanthomonas campestris (MTCC 7908), and Candida albicans (MTCC 183) were procured from Microbial Type Culture Collection and Gene Bank (MTCC; Chandigarh, India).
3
Antibacterial Activity of Plant Extract and NPs
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The Staphylococcus aureus (MTCC 737), Bacillus cereus (MTCC 1272), Listeria monocytogenes (MTCC 657), and Shigella flexneri (MTCC 1475) obtained from Microbial Type Culture Collection (MTCC, Chandigarh, India) were used for determining the antibacterial activity of the plant extract and the synthesized NPs.
4
Antimicrobial Evaluation of Leaf Extract
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The antimicrobial activity of the leaf extract was performed by selecting the food borne pathogens such as bacteria and fungi. For antibacterial assay, indicator organisms are Staphylococcus aureus (MTCC 3103), Escherichia coli (MTCC 9537), Enterobacter aerogenes (MTCC 8558), Pseudomonas aeruginosa (MTCC 10306), Klebsiella pneumoniae (MTCC 10309), Salmonella typhi (MTCC 3224), Shigella flexneri (MTCC 9543), and Bacillus subtilis (MTCC 1305) were collected from Microbial Type Culture Collection (MTCC), Chandigarh, India. Nutrient agar medium and Muller Hinton agar medium was used for maintenance of cultures and antimicrobial activity, respectively. For antifungal assay, fungal cultures are Fusarium oxysporum (NCIM 1043), Aspergillus niger (NCIM 512), Penicillium citrinum (NCIM 766), Trichoderma viridae (NCIM 1051), and Candida albicans (NCIM 3471) were collected from National Collection of Industrial Microorganisms (NCIM), Pune, India.
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