E coli atcc 25922 strain

The E. coli ATCC 25922 strain was purchased from the American Type Culture Collection (ATCC). Drug-resistant E. coli isolates were isolated from patients at the First Hospital of Jilin University (Changchun, Jilin province, China). The antibiotic susceptibility of the E. coli isolates was evaluated using the VITEK^® 2 Compact system (bioMérieux, Marcy-l’Étoile, France). Glycerol bacterial stocks were stored at -80°C and transferred to lysogeny broth (LB) medium (BioCorp, Warszawa, Poland) before recovery at 37°C. Tryptic Soy Broth (TSB) medium (bioMérieux, Marcy-l’Étoile, France) was used to culture bacterial biofilms. All strains were tested to determine the host range of the phage and to obtain the depolymerase antibiofilm activity.

The bacterial strains and plasmids used for this study are described in Table 1. The A. pleuropneumoniae strains were cultured in a brain heart infusion (BHI, Difco Laboratories, Detroit, MI, USA) medium supplemented with 10 μg/ml nicotinamide adenine dinucleotide (NAD) (Sigma-Aldrich, U.S.). For culture of A. pleuropneumoniae transconjugants (single crossovers), BHI medium was supplemented with 10 μg/ml of NAD and 7 μg/ml of chloramphenicol. E. coli ATCC 25922 strain and S. enterica ATCC 51741 strain (American Type Culture Collection, ATCC) were cultured in a Luria-Bertani (LB, Difco Laboratories, Detroit, MI, USA) medium. E. coli β2155 was grown in LB medium supplemented with 1 mM diaminopimelic acid (DAP) (Sigma-Aldrich, U.S.). All strains were routinely grown at 37°C.