Hlc 723g8 hplc analyzer

The annual number of routine PB smear examinations requested at the Department of Laboratory Medicine, Daejeon St. Mary’s Hospital (Daejeon, Republic of Korea) were 3534 in 2015, 4437 in 2016, 4677 in 2017, 4446 in 2018, and 4694 in 2019, respectively. If more than 50% abnormal or dysmorphic RBCs were observed upon the routine screening of peripheral blood smears, additional laboratory testing was performed for differential diagnosis. Briefly, an RBC autohemolysis test, RBC osmotic fragility test, and direct Coombs test were conducted to determine if hereditary elliptocytosis (HE), hereditary spherocytosis (HS), or hereditary stomatocytosis (HSt) was suspected. The high-performance liquid chromatography (HPLC) of hemoglobin (Hb) using an HLC-723G8 HPLC analyzer (Tosoh Co., Tokyo, Japan) was performed to characterize abnormal Hb variants such as HBA2, HbF, or other Hb fractions found commonly in beta thalassemia major (BTM) and beta thalassemia minor (BTm). A workflow for the screening and molecular diagnosis of suspected HHA is illustrated in Figure 1. Peripheral whole blood was collected from individuals with suspected HHA and their family members if available. Genomic DNA was extracted from peripheral blood leukocytes using a QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols.

GH was measured by a two-site chemiluminescent immunometric assay (AutoDELFIA® hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy), intra-assay CV: 5.3–6.5%, interassay CV: 5.7–6.2%, and sensitivity: up to 0.01 μg/l (0.026 mU/l).
IGF-1 was measured with the IMMULITE 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK); normal age-appropriate ranges are as follows: 1–6 years: 49–327 μg/l; 7–11 years: 57–551 μg/l; 12–13 years: 143–850 μg/l; 14–16 years: 220–996 μg/l; 17–18 years: 163–731 μg/l; 19–20 years: 127–483 μg/l; 21–35 years: 115–358 μg/l; 36–50 years: 94–284 μg/l; >50 years: 55–238 μg/l; intra-assay CV: 2.3–3.5%; interassay CV: 7.0–7.1%; and sensitivity: 20 μg/l. IGF-1 index = IGF − 1/upper limit of normal range (ULN) [24 (link)].
Insulin was measured by chemiluminescence immunoassay (ADVIA Centaur XP, Siemens, USA). BG was measured by a Hitachi 7600 Biochemical Analyzer (Tokyo, Japan). HbA_1c was detected with high-performance liquid chromatography (Tosoh HLC-723 G8 HPLC Analyzer, Japan).