Serum biochemical parameters were measured by routine automated laboratory methods. Insulin levels were measured by a commercial ELISA kit (MEA448Mu, Wuhan USCN Business Co., Ltd., Wuhan, China) according to the manufacturer’s instruction. The intra-assay coefficient of variation was 4.0%. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as described previously (Yan et al., 2017 (link)). SOD activities and MDA levels in tissues and serum were measured by the SOD assay kit (A001-3, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and MDA assay kit (A003-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively, according to the instruction manual.
Mea448mu
Manufactured by USCN
The MEA448Mu is a multi-electrode array (MEA) system. It is designed for the recording and analysis of electrical signals from in vitro cell cultures, such as neural networks. The device features 448 electrodes that can be used to monitor the activity of cells in real-time.
Automatically generated - may contain errors
2 protocols using mea448mu
1
Biochemical Parameters and Oxidative Stress
2
Metabolic Biomarkers in Mice Adiposity
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The mice were starved overnight and anesthetized two days after IPITT. Blood samples were collected and centrifuged at 3,000 rpm for 10min at 4°C to collect serum. Besides, subcutaneous white adipose tissue (WAT), perirenal WAT, epididymal WAT and liver tissue were also collected and stored at -80°C until further analysis.
Serum biochemical parameters were measured using routine automated laboratory methods. Serum levels of insulin and leptin were measured with corresponding ELISA kits (MEA448Mu, MEA084Mu, Wuhan USCN Business Co., Ltd., Wuhan, China) according to the instruction manual. Malondialdehyde (MDA) levels and Superoxide dismutase (SOD) activities in serum and tissues were detected by the MDA (A003-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and the SOD (A001-3, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) assay kit, respectively. In addition, the homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as described in a previous study [26] .
Serum biochemical parameters were measured using routine automated laboratory methods. Serum levels of insulin and leptin were measured with corresponding ELISA kits (MEA448Mu, MEA084Mu, Wuhan USCN Business Co., Ltd., Wuhan, China) according to the instruction manual. Malondialdehyde (MDA) levels and Superoxide dismutase (SOD) activities in serum and tissues were detected by the MDA (A003-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and the SOD (A001-3, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) assay kit, respectively. In addition, the homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as described in a previous study [26] .
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