For reverse western blot, Proteome Profiler Antibody Array Kits for human angiogenesis factors (R&D Systems) was used, according to the manufacturer's instructions. Factors present in LEC, MEC, or HUVEC conditioned media were compared with those in conditioned media from tumor-educated LEC (MB231-LEC), MB231-MEC, or MB231-HUVEC. Average signal (pixel density) of the pair of duplicate spots was determined using ImageJ. Expression level changes more than 200% after TCM treatment were considered as a significant perturbation. We further studied these highly changed target molecules (e.g., EGF and PDGF-BB in LEC).
Proteome profiler antibody array kits for human angiogenesis factors
Manufactured by R&D Systems
Sourced in United States
The Proteome Profiler Antibody Array Kits for human angiogenesis factors from R&D Systems are a multiplex assay designed to detect the relative abundance of 55 different human angiogenesis-related proteins in a single experiment.
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Lab products found in correlation
2 protocols using proteome profiler antibody array kits for human angiogenesis factors
1
Profiling Angiogenesis Factors in Tumor-Educated ECs
2
Angiogenesis Protein Profiling in Endothelial and Fibroblast Media
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Proteome Profiler Antibody Array Kits for human angiogenesis factors (R&D systems, MN, USA) were used to perform reverse western blots. We followed the manufacturer’s operation manual to investigate the relative levels of angiogenesis-related proteins in endothelial growth media-2 (EGM-2) and human lung fibroblast conditioned media (HLFCM).
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