Antibodies to NPC1 (#ab134113), TMEM192 (#ab186737) and PRDX4 (#ab16943) were from Abcam. Others were to CMTM6 (# HPA026980; Sigma LifeSciences), cleaved caspase-3 (#9664S; Cell Signaling Co.) and HSP70 (#sc-298; Santa Cruz Biotechnologies. Gefitinib (#13166) and 5-Fluorouracil (#14416) were from Cayman Chemicals (Ann Arbor, MI, USA). Iodoacetamide was from GE Healthcare. Camptothecin (#C9911), oxaliplatin (#O9512) drugs and PARP inhibitor (PJ34) (#P-4365), N-ethylmaleimide (NEM) were from Sigma-Aldrich. Lapatinib was from LC Laboratories, Woburn, MA and nifuroxazide was from Dr. David Frank, Dana-Farber Cancer Institute. Cell-ROX labeling (#C10422), CellTrace CFSE cell proliferation kit (#C34554) and FxCycle PI/RNase cell cycle staining solution (#F10797) were from Invitrogen. Tris(2-carboxyethyl)phosphine (TCEP), N-[6-(biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (biotin-HPDP), high-capacity streptavidin agarose beads, and Micro BCA protein assay were from Pierce. Mass spec-grade trypsin was from Promega. Control and DHHC3 shRNA and siRNA sequences and DHHC3 wild type and active site mutant reconstitution vectors were described [4 (link)]. Knockdown and reconstitution was achieved using a single vector, containing either wild type or mutant DHHC3 cDNA’s, downstream of knockdown DHHC3 shRNA [4 (link)].
Parp inhibitor pj34
Manufactured by Merck Group
PARP inhibitor (PJ34) is a laboratory research compound that functions as a poly(ADP-ribose) polymerase (PARP) inhibitor. PARP enzymes play a role in various cellular processes, and PJ34 acts to inhibit their activity. This compound is used in research settings to study the effects of PARP inhibition, without making claims about its intended use or applications.
Automatically generated - may contain errors
Lab products found in correlation
Iodoacetamide, by GE Healthcare (1 mentions)
Mass spec grade trypsin, by Promega (1 mentions)
Oxaliplatin, by Merck Group (1 mentions)
Gefitinib, by Cayman Chemical (1 mentions)
Camptothecin, by Merck Group (1 mentions)
Hsp70, by Santa Cruz Biotechnology (1 mentions)
Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions)
Cmtm6, by Merck Group (1 mentions)
5 fluorouracil, by Cayman Chemical (1 mentions)
Lapatinib, by LC Laboratories (1 mentions)
Nsc697923, by Merck Group (1 mentions)
Brefeldin a, by Thermo Fisher Scientific (1 mentions)
Etoposide, by Merck Group (1 mentions)
2 protocols using parp inhibitor pj34
1
Analyzing Protein Palmitoylation in Cancer Cells
2
Etoposide and Inhibitor Treatments
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Etoposide and inhibitors were diluted in DMSO. Etoposide (Sigma) was used at a final concentration of 50 μM, unless indicated otherwise. ATM inhibitor KU55933 (Santa Cruz) was used at 10 μM. TBK1 inhibitor MRT67307 was kindly provided by the MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, and used at 2 μM. PARP inhibitor PJ34 (Sigma) was used at 10μM. Brefeldin A (eBioscience) was used at 3μg/mL. Ubc13 inhibitor NSC697923 (Sigma) was used at 1 and 10 μM as indicated.
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