Quantitative gene expression levels were detected using real-time PCR with the ABI PRISM 7500 Fast Sequence Detection System and SYBR Advantage qPCR Premix (Clontech, 639676) according to the manufacturer’s instructions. Primers for all genes analyzed were purchased from Integrated DNA Technologies. Please see STAR Methods for Primer sequences. The relative number of copies of mRNA (RQ) was calculated based on the average Ct values using the housekeeping gene GAPDH as internal control and vehicle-treated cells as biological controls. Results are shown as mean ± SD of three independent experiments.
Abi prism 7500 fast sequence detection system
Manufactured by Takara Bio
The ABI PRISM 7500 Fast Sequence Detection System is a real-time PCR instrument designed for fast and accurate nucleic acid quantification. It features a 96-well thermal cycler and utilizes fluorescent detection technology to monitor the amplification of DNA or RNA samples in real-time.
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Lab products found in correlation
2 protocols using abi prism 7500 fast sequence detection system
1
Quantitative Gene Expression Analysis
2
Quantitative Gene Expression Analysis
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