Socs2

Manufactured by Abcam

Sourced in United States

SOCS2 is a protein that functions as a negative regulator of cytokine signaling. It is involved in the regulation of the JAK-STAT signaling pathway, a key mechanism for transmitting extracellular signals into the cell.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-SOCS2 (2 citations)

6 protocols using socs2

Signaling Protein Expression Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis and intracellular flow cytometry was performed as in (19 (link)) using antibodies to STAT3, phospho-STAT3, phospho-STAT5 (Cell Signaling Technology, Danvers, MA), STAT5a, STAT5b, SOCS1, SOCS2, SOCS3 (Abcam, Cambridge, MA) or GAPDH (EMD Millipore).

Hedl M., Sun R., Huang C, & Abraham C. (2019). STAT3 and STAT5 signaling thresholds determine distinct regulation for innate-receptor-induced inflammatory cytokines and STAT3/STAT5 disease variants modulate these outcomes. Journal of immunology (Baltimore, Md. : 1950), 203(12), 3325-3338.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Renal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following deparaffinization and hydration, 4 μm-thick renal cortical tissue sections were treated with 3% H₂O₂ for 10 minutes to inactivate endogenous enzymes. After incubation with appropriate primary antibodies (αSMA, E-cadherin, SOCS2, F4/80, TLR4, IGF1, and CD68 [Abcam, Cam-bridge, UK]) overnight at 4°C, the sections were incubated with a horseradish peroxidase-labeled goat antirabbit or goat antimouse polyclonal antibody for 30 minutes at room temperature. The sections were then counterstained with hematoxylin, dehydrated, cleared, and mounted with mounting solution. Ten fields were observed at 400× magnification with the Leica microscope. F4/80⁺ or CD68⁺ cells number and the positive integrated optical density value of each field were calculated and analyzed with Image-Pro Plus, and the mean values were compared among the experimental groups.

Li J., Dong R., Yu J., Yi S., Da J., Yu F, & Zha Y. (2018). Inhibitor of IGF1 receptor alleviates the inflammation process in the diabetic kidney mouse model without activating SOCS2. Drug Design, Development and Therapy, 12, 2887-2896.

+ Open protocol

+ Expand

Western Blot Analysis of Macrophage Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells or exosomes were collected and lysed in Laemmli buffer (Sigma, USA). The protein concentration was determined with BCA Protein Assay kit (Beyotime). Protein lysates were separated by SDS-PAGE and transferred onto NC filter membrane (Millipore). Then the NC filter membrane was subsequently incubated with the primary antibody: iNOS (Abcam), Arg1 (Abcam), p-STAT1 (Abcam), p-STAT6 (Abcam), STAT1 (Abcam), STAT6 (Abcam), SOCS2 (Abcam), PKM2 (Cell Signaling Technology, CST, Danvers, MA, USA), HIF-1α (Invitrogen, Life Technologies, Carlsbad, CA, USA), β-actin (Abcam). β-actin was used as a control. After incubation with secondary antibodies and ECL (enhanced chemiluminescence, Millipore), the protein bands were captured by Bio-Rad ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA). Quantitative analysis of protein bands was conducted with ImageJ software.

Zhang Y., Zhu L., Li X., Ge C., Pei W., Zhang M., Zhong M., Zhu X, & Lv K. (2023). M2 macrophage exosome-derived lncRNA AK083884 protects mice from CVB3-induced viral myocarditis through regulating PKM2/HIF-1α axis mediated metabolic reprogramming of macrophages. Redox Biology, 69, 103016.

+ Open protocol

+ Expand

Western Blot Analysis of Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis of skin draining LN migDC and cDC. Mice were treated every other day by intraperitonial injection of 10ng recombinant human Flt3L for twelve days prior to LN digestion and FACS sorting. For western blot analysis of protein levels in DCs following in vitro culture, Flt3L derived BMDCs were generated by culture of total bone marrow cells supplemented with 100ng/ml Flt3L for 7 days. Cultures were harvested at 0 hr or treated with 200ng/mL of IFNγ for 24 hr or 48 hr. Western blotting was conducted with the follow antibodies: SOCS2, Abcam; β–actin (AC-15), Sigma. Cells were lysed in modified RIPA buffer containing 50 mM Tris (pH 7.4), 1% NP-40,150 mM NaCl, 1 mM EDTA, 1 mM Na₃VO₄, and protease and phosphatase inhibitor cocktail (Life Technologies). Protein lysates were quantified using protein assay dye reagent (Bio-Rad). Equal amounts of proteins were separated by precast Tris-HCl SDS-polyacrylamide gel (Lonza), transferred to nitrocellulose membrane (Amersham), and incubated with primary antibodies in 1× TBS-T containing 5% non-fat dry milk overnight at 4°C. After washing the membranes in 1× TBS-T, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hr at RT, binding of the primary antibodies were detected using an enhanced chemiluminescence substrate (Life Technologies).

Nirschl C.J., Suárez-Fariñas M., Izar B., Prakadan S., Dannenfelser R., Tirosh I., Liu Y., Zhu Q., Devi K.S., Carroll S.L., Chau D., Rezaee M., Kim T.G., Huang R., Fuentes-Duculan J., Song-Zhao G.X., Gulati N., Lowes M.A., King S.L., Quintana F.J., Lee Y.S., Krueger J.G., Sarin K.Y., Yoon C.H., Garraway L., Regev A., Shalek A.K., Troyanskaya O, & Anandasabapathy N. (2017). IFNγ-Dependent Tissue-Immune Homeostasis Is Co-opted in the Tumor Microenvironment. Cell, 170(1), 127-141.e15.

+ Open protocol

+ Expand

Quantitative Protein Analysis in Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total proteins in serum, tissues and cells were evaluated by bicinchoninic acid kits (Pierce, Rockford, IL, USA). The extracted proteins that had been denatured by high temperature were mixed with loading buffer, boiled at 95 °C for 10 min, conducted with 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto the polyvinylidene fluoride membranes. After fixed with 5% bovine serum albumin (10-L16, Beijing Zhongshenglikang Technology Co., Ltd., Beijing, China), the membranes were appended with primary antibodies SOCS2 (1: 300, Abcam Inc., Cambridge, MA, USA) and GAPDH (1: 1000, Cell Signaling Technology, Beverly, MA, USA), and the horseradish peroxidase-conjugated secondary antibody (1: 5000; ab6721, Abcam). Next, the membranes were developed by enhanced chemiluminescent reagent and exposed in a dark room. GAPDH was taken as the internal reference and the data were analyzed by the Bio-Rad Image Lab system (GEL DOC EZ IMAGER, Bio-rad, CA, USA). Gray values of the protein bands were analyzed by the Image J software.

Ye J., Lin Y., Yu Y, & Sun D. (2020). LncRNA NEAT1/microRNA-129-5p/SOCS2 axis regulates liver fibrosis in alcoholic steatohepatitis. Journal of Translational Medicine, 18, 445.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from cells or tissues by using RIPA buffer (Solarbio, Beijing, China). The concentration of protein samples was assessed by a Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA). Equal amount of 30 μg protein samples were separated by sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred onto a polyvinylidene fluoride (PVDF) membrane. Then, the membrane was blocked in the non-fat milk for 2 h at room temperature and probed with primary antibodies against E-cadherin, N-cadherin, Vimentin, SOCS2, JAK2, p-JAK2, STAT5, p-STAT5 and GAPDH (Abcam, Cambridge, MA, USA) at 4 °C overnight. After incubation with goat anti-rabbit IgG-HRP secondary antibody (Abcam) at 37 °C for 2 h, the protein signaling was visualized via eyoECL plus kit (Beyotime, Shanghai, China). The original data for western blots were presented in Additional file 2.

Wang F., Wang X., Li J., Lv P., Han M., Li L., Chen Z., Dong L., Wang N, & Gu Y. (2021). CircNOL10 suppresses breast cancer progression by sponging miR-767-5p to regulate SOCS2/JAK/STAT signaling. Journal of Biomedical Science, 28, 4.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!