Mouse monoclonal anti beta actin

To detect HER2 expression, HIBEC, RBE, HuccT1, CCLP1, HGBEC, SGC-996, NOZ, GBC-SD, and EH-GB1 cells were seeded in 6-well plates at the density of 4 × 10⁵ per well. After 48 h, the total protein of each cell line was extracted. For mechanism validation, GBC-SD and EH-GB1 cells were seeded in 6-well plates at the density of 4 × 10⁵ per well until adherent and replaced with different media. Each cell line was also divided into 8 groups and treated as previously described for the cell proliferation assay. After 48 h, total protein was extracted from each cell group. Then western blotting was performed according to the standard methods. The following antibodies, Rabbit monoclonal anti-HER2 (Abcam, Catalog no. ab134182, 1:1000 dilution), Mouse monoclonal anti-GAPDH (Abcam, Catalog no. ab8245, 1:5000 dilution), Rabbit monoclonal anti-NFκB p65 (Abcam, Catalog no. ab32536, 1:10,000 dilution), Rabbit monoclonal anti-NFκB p50 (Abcam, Catalog no. ab32360, 1:10,000 dilution), Mouse monoclonal anti-beta actin (Abcam, Catalog no. ab8226, Use a concentration of 1 µg/ml), and Rabbit polyclonal anti-Lamin B1 (Abcam, Nuclear Envelope Marker, Catalog no. ab16048; Use a concentration of 0.1 µg/ml) were obtained from Abcam. Supplementary Figures 37 and 38 depicts the source data underlying Supplementary Fig. 26 and Fig. 4b, respectively.