Anti-Flag was purchased from Sigma Aldrich (F1804, Sigma Aldrich, Saint Louis, MO, USA). Voltage-dependent anion channel (VDAC) (D73D12) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Mic60 (ab137057), Mic10 (ab84969), CHCHD3 (ab224565), translocase of the outer membrane 20 (Tom20) (ab56783), heat shock protein 60 (Hsp60) (ab46798), glyceraldehydes-3-phosphate dehydrogenase (ab181602), β-actin (ab8226), and tyrosine hydroxylase (TH) (ab112) were from Abcam (Cambridge, UK). The anti-mouse and anti-rabbit secondary antibodies (1:1000) were purchased from Cell Signaling Technology. MitoTracker Deep Red was obtained from Life Technologies (Carlsbad, CA, USA) (M22426). 1-methyl-4-phenyl-1,2,3,6-tetrahydropyri-dine (MPTP) hydrochloride and methyl-4-phenylpyridi-nium (MPP+) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
1 methyl 4 phenyl 1 2 3 6 tetrahydropyri dine mptp hydrochloride
Manufactured by Santa Cruz Biotechnology
Sourced in United States
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride is a chemical compound. It is a neurotoxin that can selectively destroy dopaminergic neurons in the substantia nigra region of the brain.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag, by Merck Group (1 mentions)
Mitotracker deep red, by Thermo Fisher Scientific (1 mentions)
Ab56783, by Abcam (1 mentions)
Ab46798, by Abcam (1 mentions)
Ab84969, by Abcam (1 mentions)
Ab224565, by Abcam (1 mentions)
Anti mouse and anti rabbit secondary antibodies, by Cell Signaling Technology (1 mentions)
Ab181602, by Abcam (1 mentions)
Rabbit anti 4 hne, by Alpha Diagnostic (1 mentions)
Glial fibrillary acidic protein (gfap), by Thermo Fisher Scientific (1 mentions)
Mouse anti th, by Merck Group (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Anti mouse rabbit hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
2 protocols using 1 methyl 4 phenyl 1 2 3 6 tetrahydropyri dine mptp hydrochloride
1
Mitochondrial Protein Detection Protocol
2
Immunohistochemical Analysis of MPTP-Induced Parkinson's
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The primary antibodies used in this study included mouse anti-TH (Millipore, Temecula, CA, USA, MAB318), rabbit anti-GAPDH (Cell Signaling, Danvers, MA, USA, 2118), rabbit anti-4-HNE (Alpha Diagnostics, San Antonio, TX, USA, HNE11-S), GFAP (Invitrogen, Rockford, IL, USA, PA5-16291), and iba1 (Wako, Osaka, Japan, 019-19741). Secondary antibodies used in this study included anti-mouse/rabbit HRP-linked secondary antibody (Cell Signaling, Danvers, MA, USA, 7076 and 7074) and goat anti-mouse or goat anti-rabbit (Millipore, Temecula, CA, USA, AP124 or AP132) peroxidase-conjugated antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) hydrochloride (Santa Cruz, CA, USA, 23007-85-4) was purchased and dissolved in saline for mice treatment.
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