AFOG staining was performed on paraffin sections following the manufacturer’s instructions51 (link). The sections were incubated in Bouin’s solution (HT10132; Sigma) at 56 °C for 2.5 h, and at room temperature for 1 h, washed in tap water, incubated in 1% phosphomolybdic acid (P4869; Sigma) for 5 min, washed with water, and then stained with AFOG solution consisting of 3 g acid fuchsin (F8129; Sigma), 2 g orange G (O3756, Sigma), and 1 g aniline blue (AB0083; BBI) dissolved in 200 ml acidified distilled water (pH 1.1) for 10 min. The sections were rinsed with distilled water, dehydrated, mounted, and staining was photographed under a DM5000B microscope (Leica, Germany).
F8129
Manufactured by Merck Group
Sourced in United Kingdom
F8129 is a laboratory equipment product offered by Merck Group. It serves as a general-purpose centrifuge for separating components of a liquid mixture based on their density differences.
Automatically generated - may contain errors
2 protocols using f8129
1
AFOG Staining Protocol for Paraffin Sections
2
Histological Analysis of Tendon Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The mid‐substance portions of the tendon samples in experimental group and control group were placed in 10% phosphate‐buffered formalin solution (G2161, Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) at room temperature for 4 h, and then were processed for histological analysis. The samples were embedded in paraffin and microtomed to obtain 5 μm‐thick longitudinal sections. The sections were attached to glass slides, stained with Van Gieson's trichrome (F8129, Sigma‐Aldrich, London, UK), and observed under BX53 optical microscopy and photomicrographs were taken (Olympus, Tokyo, Japan).
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