Apoptosis and necrosis were observed in the control MCF-7 cells versus the treated ones using flow cytometry via the Annexin V-FITC Kit (BD PharmingenTM, San Diego, CA, USA). Cells were collected, washed in PBS, then labeled with FITC-conjugated Annexin V and PI (Invitrogen, Carlsbad, CA, USA). The samples were analyzed with the BD AccuriTM C6 flow cytometer (San Jose, CA, USA).
Fitc conjugated annexin 5 and pi
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated Annexin V and PI is a laboratory reagent used for the detection and analysis of apoptosis (programmed cell death) in cells. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, which is exposed on the surface of cells undergoing apoptosis. FITC (Fluorescein Isothiocyanate) is a fluorescent dye that is conjugated to Annexin V, allowing for the visualization of apoptotic cells. Propidium Iodide (PI) is a DNA-binding dye that is used to stain cells with compromised cell membranes, typically associated with late-stage apoptosis or necrosis.
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2 protocols using fitc conjugated annexin 5 and pi
1
Apoptosis and Necrosis Quantification
2
Annexin V-FITC Apoptosis Assay
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In 6-well plates, the processed HCAECs were rinsed in PBS. After trypsinization, the cells were resuspended in binding buffer (100 μL). FITC-conjugated Annexin V and PI (1 μL, Invitrogen, Carlsbad, CA, USA) were added to the binding buffer. After 15 minutes, apoptotic cells were analyzed by flow cytometry (Agilent, USA).
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