Human smooth muscle cell growth medium 2

Male Sprague Dawley rats were killed by cervical dislocation in accordance with schedule 1 of the U.K. Animals (Scientific Procedures) Act 1986 and Directive 2010/63/EU of the European Parliament and with the approval of the University of Bristol. Methods used to culture VSMCs and ECs are described in detail in the Supplement. Human aortic VSMCs (HuVSMCs) and human umbilical endothelial cells (HUVC) at passage 2–8 were purchased from Promocell. All experiments were replicated for the number of time shown in the text and figures using different batches of cells that were prepared from different animals/donors. Cultures of rat aortic VSMCs (RaVSMCs) were prepared as previously described [22 (link)] and cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% foetal bovine serum, 100 U/ml penicillin/streptomycin and 2.5 mM l-glutamine. Human VSMC were cultured in human smooth muscle cell growth medium 2 (Promocell), supplemented with the supplement pack (Promocell), 100 U/ml penicillin/streptomycin and 2.5 mM l-glutamine. HUVEC were cultured in Endothelial Cell Growth Medium 2 supplemented with the supplied supplement pack (Promocell), 100 U/ml penicillin/streptomycin and 2.5 mM l-glutamine.