ChIP was performed using the ChIP-IT express kit (Active Motif) according to Manual's Instructions. The protocol was erformed on RNAi treated HeLa cells and using 10 μl of H3K27me2/3 antibody (Active Motif, 39535) and digoxin as a negative control (Jackson Laboratories, 200-002-156). After ChIP, DNA was purified using Phenol Chloroform and ethanol precipitation with glycogen. DNA concentration was measured using Qubit (High Sensitivity Kit, Thermo) and reduced to 0.2 ng μl. qPCR was quantified using SYBR green Master Mix (Thermo Scientific) and according to manufacturer's instructions (primers in Supplementary Table 2 ). Delta–delta CT method was used with normalization for Input DNA. MYT1 gene was used as a control for H3K27me2/3 enrichment.
H3k27me2 3 antibody
Manufactured by Active Motif
The H3K27me2/3 antibody is a specific antibody that recognizes the di- and tri-methylated forms of histone H3 at lysine 27. This antibody is used in various research applications, such as chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence, to study the epigenetic regulation of gene expression.
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Lab products found in correlation
2 protocols using h3k27me2 3 antibody
1
Profiling H3K27me2/3 Occupancy via ChIP
2
Histone H3K27 Methylation ChIP-qPCR
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H3K27me2/3 ChIP using -H3K27me2/3 antibody (Active Motif, 39536), which recognizes di-or trimethylated H3K27, was performed as previously described (Wiles et al. 2020) . The isolated DNA was used for qPCR (see Supplemental File 2 for primers) or prepared for sequencing (Wiles et al. 2020) . Sequencing was performed by the University of Oregon Genomics and Cell Characterization Core Facility.
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