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2 protocols using terutroban

1

Vascular Reactivity Reagents Protocol

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Urethane, norepinephrine bitartrate, phenylephrine, sodium nitroprusside, acetylcholine, dimethyl sulfoxide (DMSO), indomethacin, ethylenediaminetetraacetic acid (EDTA), terutroban, acetylsalicylic acid, 1-benzylimidazole, and kaempferol were purchased from Sigma-Aldrich (Prague, Czech Republic). The Krebs solution salts and 96% ethanol were purchased from Penta s.r.o. (Prague, Czech Republic). Arachidonic acid was bought from Medista (Prague, Czech Republic). Heparin sodium was purchased from Zentiva (Prague, Czech Republic); U-46619, thromboxane B2 ELISA kit, prostaglandin H2, and the COX inhibitor screening kit were from the Cayman Chemical Company (Ann Arbor, MI, USA). Collagen was obtained from Diagnostica a.s. (Prague, Czech Republic), and saline from B. Braun (Prague, Czech Republic).
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2

Effects of Antiplatelet and Anticoagulant Agents on Platelet Function

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ASA, indomethacin, ethylenediaminetetraacetic acid (EDTA), 1-benzylimidazole and terutroban were purchased from Sigma Aldrich (Prague, Czech Republic). U-46619, a thromboxane B2 ELISA kit, prostaglandin H2 and a cyclooxygenase (COX) inhibitor screening assay kit were obtained from the Cayman Chemical Company (Ann Arbor, MI, USA). Collagen was purchased from Diagnostica a.s. (Prague, Czech Republic) and heparin sodium from Zentiva (Prague, Czech Republic). AA was obtained from Roche (Prague, Czech Republic) and sodium chloride (0.9%) from B. Braun (Prague, Czech Republic); dimethyl sulfoxide (DMSO) and 96% ethanol were bought from Penta (Prague, Czech Republic). Human control plasma, prothrombin time reagent (Technoplastin® HIS), activated partial thromboplastin time (aPTT) reagent (DAPTTIN®) and calcium chloride were purchased from Technoclone (Vienna, Austria).
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