Dimethyl sulfoxide dmso for molecular biology

The synthesis, spectral and photophysical characteristics of hyperbranched polymers S1 and S2 presented in Scheme 1 were described recently [20 (link)]. The same polymers were used to prepare the copper complexes in this work. Dimethyl sulfoxide (DMSO) for molecular biology and Cu(NO₃)₂·3H₂O were used as obtained from Sigma-Aldrich (Darmstadt, Germany). The used methods for characterization and microbiological activity of polymers are presented in the Supplementary Materials.

8-(4-chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (D-007) was purchased from Biolog Life Science Institute, Bremen, Germany. 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM), Alamar blue cell viability reagent and SYPRO™ Orange protein stain were purchased from ThermoFisher Scientific, Waltham, MA, USA. Adenosine 3′,5′-cyclic monophosphate (cyclic AMP) sodium salt, 0.33% neutral red solution, dimethyl sulfoxide (DMSO) for molecular biology and anti-Rabbit IgG, HRP conjugate, were obtained from Sigma-Aldrich, St. Louis, MO, USA. Rap1A/Rap1B (26B4) rabbit mAb, EPAC1 (DE3) mouse mAb and STAT3/phospho-STAT3 (Tyr 705) antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA.

Analytical-grade hexane, ethyl acetate and methanol (POCh, Gliwice, Poland) were used for the extraction and isolation studies. Chromatographic grade methanol, acetonitrile, water, ammonium formate and formic acid (J.T. Baker, Deventer, The Netherlands) were used for the HPLC-DAD and HPLC-ESI-QTOF-MS/MS analyses. Deuterated chloroform (CDCl₃, 99.8 atom %D) for NMR analyses was acquired from Armar Chemicals (Döttingen, Switzerland). Water was purified using a Simplicity^® water purification system (Millipore, Grenoble, France). Dimethyl sulfoxide (DMSO) for molecular biology (Sigma-Aldrich, Darmstadt, Germany). E3 solution for Danio rerio experiments (solution of a mixture of salt: NaCl, KCl, CaCl₂, MgCl₂).