The following antibodies were used for western blotting: mouse anti-PrP [1:10,000, POM19, amino acids 201–225; POM1 (Polymenidou et al., 2008 (link))]; anti-beta actin (1:5000, Genetex); anti-GAPDH (1:5000, Novus Biologicals #NB300–221SS); anti-αtubulin (1:1000, Cell Signaling Technologies #3873); anti-synapsin I (1:10,000, Fisher Scientific #AB1543MI); anti-synaptophysin (1:10,000, Invitrogen #MA1213); anti-phosphorylated synapsin I (1:1000; Cell Signaling Technology; #2311); anti-PSD-95 (1:5000, Cell Signaling Technology #3450); anti-SNAP25 (1:10,000, Cell Signaling Technology #5308); anti-mGluR5 (1:5000, Cell Signaling Technology #55920); anti-phosphorylated GluA1-S845 (1:1000, Cell Signaling Technology #8084); anti-GluA1 (1:1000, Cell Signaling Technology #13185); anti-Arc (1:2000, Proteintech #16290–1-AP); anti-EGR1 (1:1000, Cell Signaling Technology #4154); anti-EGR2 (1:1000, Novus Biologicals #NB110–59723SS); anti-Homer1 (1:1000, Cell Signaling Technology #8231); anti-GluN1 (1:1000, Cell Signaling Technology #5704).
Anti mglur5
Manufactured by Cell Signaling Technology
Anti-mGluR5 is a primary antibody that specifically binds to the metabotropic glutamate receptor 5 (mGluR5). mGluR5 is a G protein-coupled receptor that plays a role in neuronal signaling and synaptic function.
Automatically generated - may contain errors
Lab products found in correlation
Anti synaptophysin, by Thermo Fisher Scientific (2 mentions)
Anti glua1, by Cell Signaling Technology (2 mentions)
Anti glun1, by Cell Signaling Technology (2 mentions)
Anti psd95, by Cell Signaling Technology (2 mentions)
Anti phosphorylated synapsin 1, by Cell Signaling Technology (2 mentions)
Anti phosphorylated glua1 s845, by Cell Signaling Technology (2 mentions)
Anti arc, by Proteintech (2 mentions)
Anti egr2, by Novus Biologicals (2 mentions)
Anti homer1, by Cell Signaling Technology (2 mentions)
Anti gapdh, by Novus Biologicals (2 mentions)
Anti synapsin 1, by Thermo Fisher Scientific (2 mentions)
Anti α tubulin, by Cell Signaling Technology (2 mentions)
Anti snap25, by Cell Signaling Technology (2 mentions)
Anti egr1, by Cell Signaling Technology (2 mentions)
Anti beta actin, by GeneTex (2 mentions)
Anti glun2a, by Merck Group (1 mentions)
Pierce ecl plus, by Thermo Fisher Scientific (1 mentions)
Anti glua1, by Merck Group (1 mentions)
Anti glua2, by Merck Group (1 mentions)
Anti glun2b, by BD (1 mentions)
3 protocols using anti mglur5
1
Comprehensive Antibody Panel for Western Blotting
2
Quantifying Hippocampal Glutamate Receptor Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hippocampi were collected in RIPA buffer (25 mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% SDS). The samples were clarified by centrifugation at 14000 g and the protein concentration was determined using bicinchoninic acid assay kit (BCA; Thermo Scientific). Samples were heated for 10 min at 70°C. 10 μg of protein for each sample was loaded on a 7% SDS-polyacrylamide gel, transferred to a nitrocellulose membrane. After blocking with 5% non-fat milk, membranes were probed overnight with anti mGluR5 (1/1000, Cell Signaling), anti-GluA1 (1/500, Merck), anti-GluA2 (1/1000, Merck), anti GluN2A (1/250, Merck), anti-GluN2B (1/500, BD Biosciences) or anti-β tubulin (1/10000, Neuromics). Membranes were then incubated with secondary antibodies coupled to peroxidase (Dako) and peroxidase was detected with Pierce ECL Plus (Thermo Scientific) on ECL hyperfilm.
3
Comprehensive Antibody Panel for Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for western blotting: mouse anti-PrP [1:10,000, POM19, amino acids 201–225; POM1 (Polymenidou et al., 2008 (link))]; anti-beta actin (1:5000, Genetex); anti-GAPDH (1:5000, Novus Biologicals #NB300–221SS); anti-αtubulin (1:1000, Cell Signaling Technologies #3873); anti-synapsin I (1:10,000, Fisher Scientific #AB1543MI); anti-synaptophysin (1:10,000, Invitrogen #MA1213); anti-phosphorylated synapsin I (1:1000; Cell Signaling Technology; #2311); anti-PSD-95 (1:5000, Cell Signaling Technology #3450); anti-SNAP25 (1:10,000, Cell Signaling Technology #5308); anti-mGluR5 (1:5000, Cell Signaling Technology #55920); anti-phosphorylated GluA1-S845 (1:1000, Cell Signaling Technology #8084); anti-GluA1 (1:1000, Cell Signaling Technology #13185); anti-Arc (1:2000, Proteintech #16290–1-AP); anti-EGR1 (1:1000, Cell Signaling Technology #4154); anti-EGR2 (1:1000, Novus Biologicals #NB110–59723SS); anti-Homer1 (1:1000, Cell Signaling Technology #8231); anti-GluN1 (1:1000, Cell Signaling Technology #5704).
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