Sulfuric acid, sodium hydroxide, acetonitrile (Ultra high performance liquid chromatography (UHPLC) grade), ethylenediaminetetraacetic acid (EDTA), sodium borate, and hydrochloric acid were obtained from Panreac (Castelar del Vallés, Barcelona, Spain). Sodium acetate (anhydrous), trimethylamine (TEA), phosphoric acid, ammonium molybdate, and methyl cellulose were purchased from Sigma-Aldrich (Darmstadt, Germany). Standard of amino acids (L-arginine (Arg), L-alanine (Ala), L-asparagine (Asn), L-aspartic acid (Asp), glycine (Gly), L-glutamic acid (Glu), L-glutamine (Gln), L-histidine (His), L-isoLeucine (Ileu), Leucine (Leu), L-Lysine (Lys), L-norvaline (Nor), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-tryptophan (Trp), L-tyrosine (Tyr), and L-valine (Val)) were purchased from Sigma-Aldrich (Steinheim, Germany). All the other chemicals and reagents used were of analytical grade.
Sodium borate
Manufactured by ITW Reagents
Sourced in Germany
Sodium borate, also known as borax, is a mineral compound that consists of sodium, boron, and oxygen. It is a white, crystalline solid that is widely used in various industrial and laboratory applications. Sodium borate's core function is as a chemical agent, providing a source of boron and sodium in chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Toluidine blue, by Merck Group (2 mentions)
Ammonium molybdate, by Merck Group (1 mentions)
Sulfuric acid, by ITW Reagents (1 mentions)
Leucine, by Merck Group (1 mentions)
Acetonitrile, by ITW Reagents (1 mentions)
Sodium hydroxide (naoh), by ITW Reagents (1 mentions)
Hydrochloric acid (hcl), by ITW Reagents (1 mentions)
L norvaline, by Merck Group (1 mentions)
L tryptophan, by Merck Group (1 mentions)
L histidine, by Merck Group (1 mentions)
L phenylalanine, by Merck Group (1 mentions)
L asparagine, by Merck Group (1 mentions)
L threonine, by Merck Group (1 mentions)
L valine, by Merck Group (1 mentions)
L isoleucine, by Merck Group (1 mentions)
L serine, by Merck Group (1 mentions)
L alanine, by Merck Group (1 mentions)
L tyrosine, by Merck Group (1 mentions)
L arginine, by Merck Group (1 mentions)
L proline, by Merck Group (1 mentions)
3 protocols using sodium borate
1
Amino Acid Analysis by UHPLC
2
Ultrastructural Analysis of Cortical Regions
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Sections containing BA24, vBA38, dBA38, and BA21 were selected, washed in 0.1 M PB and postfixed for 24 h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK), and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma, C0250-500G, Germany) buffer (0.1 M). The sections were treated with 1% OsO4 (Sigma, O5500, Germany), 0.1% potassium ferrocyanide (Probus, 23,345, Spain), and 0.003% CaCl2 in sodium cacodylate buffer (0.1 M) for 1 h at room temperature. They were then stained with 1% uranyl acetate (EMS, 8473, USA), dehydrated, and flat-embedded in Araldite (TAAB, E021, UK) for 48 h at 60°C (DeFelipe and Fairén 1993 (link)). The embedded sections were then glued onto a blank Araldite block. Semithin sections (1–2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac, 141644, Spain). The last semithin section (which corresponds to the section immediately adjacent to the block surface) was examined under light microscope and photographed to accurately locate the neuropil regions to be examined (Fig. 1 ).
3
Transmission Electron Microscopy Tissue Preparation
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EC sections were post-fixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl 2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma, C0250-500G, Germany) buffer (0.1M). These sections were washed in sodium cacodylate buffer (0.1M) and treated with 1% OsO 4 (Sigma, O5500, Germany), 0.1% potassium ferrocyanide (Probus, 23345, Spain) and 0.003% CaCl 2 in sodium cacodylate buffer (0.1M) for 1h at room temperature. After (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
washing in PB, sections were stained with 2% uranyl acetate (EMS, 8473, USA), and then dehydrated and flat-embedded in Araldite (TAAB, E021, UK) for 48h at 60ºC (DeFelipe and Fairén, 1993). Embedded sections were glued onto a blank Araldite block and trimmed. Semithin sections (1-2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac, 141644, Spain). The last semithin section (which corresponds to the section immediately adjacent to the block surface) was examined under light microscope and photographed to accurately locate the neuropil regions to be examined (Fig. 2).
washing in PB, sections were stained with 2% uranyl acetate (EMS, 8473, USA), and then dehydrated and flat-embedded in Araldite (TAAB, E021, UK) for 48h at 60ºC (DeFelipe and Fairén, 1993). Embedded sections were glued onto a blank Araldite block and trimmed. Semithin sections (1-2 μm thick) were obtained from the surface of the block and stained with 1% toluidine blue (Merck, 115930, Germany) in 1% sodium borate (Panreac, 141644, Spain). The last semithin section (which corresponds to the section immediately adjacent to the block surface) was examined under light microscope and photographed to accurately locate the neuropil regions to be examined (Fig. 2).
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