Rpmi 1640 medium powder

LH was bought from National Institutes of Food and Drug Control of China. RPMI-1640 medium powder, 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT), 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), menadione, morpholinepropanesulfonic acid (MOPS), and dibutyryl-cAMP (db-cAMP) were bought from Sigma-Aldrich (Shanghai, China). LH was dissolved in DMSO and stored at -20°C.
C. albicans SC5314, C. albicans ATCC90028, Candida glabrata ATCC2001, Candida parapsilosis ATCC22019, and Candida tropicalis ATCC7349 bought from China General Microbiological Culture Collection Center (CGMCC) were maintained on yeast extract-peptone-dextrose (YPD) agar medium (1% yeast extract, 2% peptone, 2% dextrose, and 2% agar). Before each test, a colony was picked up and transferred into 5 mL YPD medium in a sterile tube and incubated overnight at 28°C with rotation (140 rpm).

Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, C2920), antimycin A (A8674), rotenone (R8875), monensin sodium salt (monensin, M5273), 2-deoxyglucose (2-DG, D6134), Hoechst 33342 (Hoechst, B2261), Concanavalin A (C2010), lipopolysaccharides (LPS) from Escherichia coli (L2637), bovine serum albumin (BSA, A6003), Triton-X 100 (T8787), sodium chloride (S9888) and Roswell Park Memorial Institute (RPMI) 1640 medium powder without phenol red and HEPES (R8755) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). RPMI 1640 medium without phenol red and HEPES (11835030), Dulbecco's phosphate-buffered saline (DPBS, 14190094), Hanks’ Balanced Salt Solution (HBSS, 14175095) and penicillin–streptomycin (15140122) were purchased from Thermo Fisher Scientific (Pittsburgh, Pennsylvania, USA). XF RPMI assay medium pH 7.4 (103576–100), XF 1.0 M glucose (103576–100), XF 100 mM pyruvate (103578–100) and XF 200 mM glutamine (103579–100) were purchased from Seahorse Biosciences, Agilent Technologies (Santa Clara, California, USA). Interleukin 4 (IL4, 214-14) and interferon-gamma (IFNγ, 315-05) were purchased from Peprotech (London, UK).