The reagents used in this study were as follows: MEK inhibitor PD0325901 (S1036) and GSK-3 inhibitor CHIR99021 (S2924) were purchased from Selleckchem (Houston, TX), ERK1/2 inhibitor SCH772984 (HY-50846) from MCE (Monmouth Junction, NJ), MG132 (M8699) from Sigma (St. Louis, MO) and Actinomycin D (HY-17559) from MCE(Monmouth Junction, NJ). The antibodies used in this study were as follows: Erk1/2 (4695), P-ERK1/2 (4370), E2F1 (3742) and SP1 (9389) from Cell Signaling Technology (Danvers, MA); DNMT1 (sc-20701) and DNMT3A (sc-20703) from Santa Cruz Biotechnology (Dallas, TX); UHRF1 (21402-1-AP) from Proteintech (Rosemont, IL); β-actin (M1210-2) from HUABIO (Cambridge, MA) and GAPDH (3B3) from Abmart (Berkeley Heights, NJ).
β actin m1210 2
Manufactured by Huabio
β-actin (M1210-2) is a lab equipment product. It is a protein that plays a role in various cellular processes. The core function of this product is to serve as a structural component and contribute to the organization and dynamics of the cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
Mg132, by Merck Group (2 mentions)
Chir99021, by Selleck Chemicals (1 mentions)
Dnmt3a sc 20703, by Santa Cruz Biotechnology (1 mentions)
Pd0325901, by Selleck Chemicals (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti rabbit igg a0208, by Beyotime (1 mentions)
Amersham imager 680, by GE Healthcare (1 mentions)
Ab32043, by Abcam (1 mentions)
Amersham imager, by Cytiva (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Alexa fluor555 phalloidin a34055, by Thermo Fisher Scientific (1 mentions)
Ubiquitin, by Santa Cruz Biotechnology (1 mentions)
E cadherin 610182, by BD (1 mentions)
α tubulin 11224 1 ap, by Proteintech (1 mentions)
4 protocols using β actin m1210 2
1
Investigating ERK/MAPK Signaling Modulators
2
Western Blot Analysis of Signaling Proteins
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Cells were lysed in 50 mM Tris, 150 mM NaCl, 1% Triton X-100, and 1 mM EDTA with pH 7.4. Lysates were mixed with protein loading buffer, boiled, and centrifuged. 10 μl of the cell lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane (Millipore), then probed with specific antibodies, and antibody binding detected by chemiluminescence. Antibodies for HA (0906–1), Flag (M1403-2) and β-actin (M1210-2) were purchased from HUABIO at 1:5000. Antibody for RNAP (sc-101597) was purchased from Santa Cruz at 1:1000. Tubulin (AF1216) and calnexin (AC018) antibodies were obtained from Beyotime Biotechnology at 1:1000. Anti-His antibody (AB102‐02; TianGen Biotech) or anti-MBP antibody (A00190‐100; Genscript Technology) was used as primary antibodies for immunoblotting. Antibodies for IκBα (4814), Erk1/2 (9107), phospho-Erk1/2 (9101s), p38α (9218), phospho-p38α (9215s), JNK (9252), phospho-JNK (9251S), phospho-MEK1/2 (9121), phospho-MKK3/6 (9231S), phospho-MKK4 (9156S), phospho-MKK7 (4171S) and phospho-ASK1 (3765) were purchased from Cell Signaling Technology at 1:1000. ASK1 antibody (ET1608-54) was from HUABIO at 1:1000. HRP-conjugated goat anti-mouse (A0216) and anti-rabbit IgG (A0208) antibodies were purchased from Beyotime Biotechnology at 1:5000.
3
STAT5A Protein Detection by Western Blot
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Cells were lysed in SDS sample buffer and denatured by heating on 100°C for 10 minutes. Proteins were separated on 10% Bis-Tris polyacrylamide gels. Western blot images were captured by Amersham Imager 680 (GE Healthcare). Antibody for STAT5A (ab32043) was purchased from Abcam. β-actin (M1210-2) was purchased from HuaBio.
4
Antibodies and Reagents for Cell Signaling Research
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Antibodies against human CD147 was produced by our lab; Par3 (07‐330) and integrin α5 (MAB1956Z) were obtained from Merck Millipore (Darmstadt, Germany); β‐catenin (51067‐2‐AP), occludin (13409‐1‐AP), lamin B (66045‐1‐AP), α‐tubulin (11224‐1‐AP), and Src (60315‐1‐AP) were purchased from Proteintech (Wuhan, China); phosphotyrosine (ARR8004) was obtained from Antibody Revolution (South San Francisco, CA); E‐cadherin (ET1607‐75) and β‐actin (M1210‐2) were purchased from Huabio (Hangzhou, China); multidrug resistance–associated protein 2 (MRP2, ab3373) and mouse CD147 (ab34016) were obtained from Abcam (Cambridge, UK); ZO‐1 (sc‐10804), integrin β1 (sc‐13590), phosphorylated Src (Tyr416, sc‐101802), and ubiquitin (sc‐271289) were purchased from Santa Cruz Biotechnology (Dallas, TX); Na+/taurocholate cotransporting polypeptide (orb13624), mouse MRP2 (orb11075), and CBLL1/Hakai (orb2538) were obtained from Biorbyt (Cambridge, UK); E‐cadherin (610182) was from BD Biosciences (Franklin Lakes, NJ); Alexa Fluor 555 phalloidin (A34055) was from Invitrogen (Carlsbad, CA); CellLight Lysosomes‐RFP (C10597) was purchased from Cell Signaling Technology; Src family activator (sc‐3052) was purchased from Santa Cruz Biotechnology; Src kinase inhibitor Src I‐1, MG132, and chloroquine were from Sigma (St. Louis, MO); TGF‐β1 (100‐21‐10VG) was obtained from Peprotech (Rocky Hill, NJ).
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