The in vitro release of PTX from the block copolymer NPs was studied in pH-adjusted release media (pH ~7.4 and ~5.0) at 37 °C. Aliquots (500 µL) of drug-loaded block copolymer NPs in PBS were loaded into 36 Slide-A-Lyzer MINI dialysis microtubes with MWCO 10,000 (Pierce, Rockford, IL, USA). These microtubes were dialyzed against 4 L of pH-adjusted PBS buffer gently stirred. The drug release experiments were done in triplicate. At each sampling time, it was removed three microtubes from the dialysis system, and 300 µL from each microtube was sampled and diluted to 1.0 mL by using Acetonitrile (Lach-ner, Czech Republic). The PTX content at each sampling time was then determined via HPLC by applying the same procedure used to determine LC and LE.
Acetonitrile
Manufactured by Lach-Ner
Sourced in Czechia
Acetonitrile is a colorless, volatile, and highly flammable organic solvent commonly used in various laboratory applications. It has a distinctive odor and is miscible with water and many organic solvents. Acetonitrile serves as a core component in analytical techniques, chemical reactions, and extraction processes conducted in scientific laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform, by Lach-Ner (2 mentions)
Acetonitrile, by Shimadzu (1 mentions)
Chromolith performance rp 18e, by Shimadzu (1 mentions)
High performance liquid chromatography (hplc), by Shimadzu (1 mentions)
Allyl bromide, by Merck Group (1 mentions)
Diisopropylamine, by Merck Group (1 mentions)
Dichloromethane, by Lach-Ner (1 mentions)
Methyl 4 nitrobenzenesulfonate, by Merck Group (1 mentions)
Ethylene carbonate, by Merck Group (1 mentions)
Tetrabutylammonium perchlorate, by Merck Group (1 mentions)
Dimethyl carbonate, by Merck Group (1 mentions)
N methyl 2 pyrrolidone, by Merck Group (1 mentions)
5 protocols using acetonitrile
1
In Vitro PTX Release from Block Copolymer NPs
2
Synthesis and Characterization of Copolymers
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Chloroform (spectroscopic grade), acetonitrile (extra dry), tetrabutylammonium hexafluorophosphate (electrochemical grade), and silver nitrate (AgNO3) were purchased from commercial suppliers (Lach-Ner, Ltd., Neratovice, Czech Republic; VWR International s.r.o., Stříbrná Skalice, Czech Republic; Merck spol. s.r.o., Praha, Czech Republic; Sigma Aldrich spol. s.r.o., Praha, Czech Republic).
The copolymers CEHCz-EHPDI and CFC8-EHPDI under study were synthesized by the Suzuki coupling reaction. The synthesis and their characterization are described in our previous paper [53 (link)]. The weight-average molecular weight (Mw) and dispersity (Đ) were for CEHCz-EHPDI Mw = 28,570, Đ = 2.04 and for CFC8-EHPDI Mw = 13,230, Đ = 1.39.
The copolymers CEHCz-EHPDI and CFC8-EHPDI under study were synthesized by the Suzuki coupling reaction. The synthesis and their characterization are described in our previous paper [53 (link)]. The weight-average molecular weight (Mw) and dispersity (Đ) were for CEHCz-EHPDI Mw = 28,570, Đ = 2.04 and for CFC8-EHPDI Mw = 13,230, Đ = 1.39.
3
PTX Quantification in Drug-Loaded Nanoparticles
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PTX loaded into the NPs was measured by HPLC (Shimadzu, Japan) using a reverse-phase column Chromolith Performance RP-18e (100 × 4.6 mm2, eluent water-acetonitrile with acetonitrile gradient 0–100 vol %, flow rate = 1.0 mL·min−1). Firstly 100 µL of the drug-loaded NPs was collected from the bulk sample, filtered (0.45 µm), and diluted to 900 µL with acetonitrile (Lach-ner, Neratovice, Czech Republic). Such procedure led to NPs dissolution. Afterward, 20 µL of the final sample was injected through a sample loop. PTX was detected at 227 nm using ultraviolet (UV) detection. The drug-loading content (LC) and the drug-loading efficiency (LE) were calculated by using the following equations:
4
Synthesis and Deposition of Poly(2-oxazoline) on PTFE
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Methyl 4-nitrobenzenesulfonate, diisopropylamine, allyl bromide and butyllithium in hexanes (2.5 M) were purchased from Sigma-Aldrich (Steinheim, Germany). 2-Methyl-2-oxazoline (MeOx) provided by TCI (TCI Europe N.V., Belgium) was dried over KOH for 48 h, distilled over CaH2 under the reduced pressure and stored under argon. Acetonitrile (J.T. Baker, Netherlands, HPLC grade) and dichloromethane (Lach-Ner, Czech Republic) were distilled over CaH2 and stored on molecular sieves under argon. Tetrahydrofuran (Centralchem, Slovakia) was distilled from sodium benzophenone ketyl prior to use. Isopropanol (Penta s.r.o., Czech Republic) and chloroform (Lach-Ner, Czech Republic) were used as received. For washing of samples before and after plasma treatment deionized water was used.
Polytetrafluoroethylene (PTFE) sheets with a thickness of 1 mm provided by Goodfellow Cambridge Ltd. were used as a substrate material for the deposition of the poly(2-oxazoline) layer. Before experiments, PTFE sheets were cut into different shapes appropriate for individual measurement techniques (squares with dimensions of 1 × 1 cm2, rectangles with dimensions of 1 × 2.5 cm2, circular samples with a diameter of 15 mm). Before all experiments, PTFE samples were ultrasonically cleaned in isopropanol and distilled water, in both solvents 2 times for 10 min and then dried in the nitrogen flow.
Polytetrafluoroethylene (PTFE) sheets with a thickness of 1 mm provided by Goodfellow Cambridge Ltd. were used as a substrate material for the deposition of the poly(2-oxazoline) layer. Before experiments, PTFE sheets were cut into different shapes appropriate for individual measurement techniques (squares with dimensions of 1 × 1 cm2, rectangles with dimensions of 1 × 2.5 cm2, circular samples with a diameter of 15 mm). Before all experiments, PTFE samples were ultrasonically cleaned in isopropanol and distilled water, in both solvents 2 times for 10 min and then dried in the nitrogen flow.
5
Synthesis of Ca1.0Si1.0Ge1.0 Crystals
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The crystals of Ca1.0Si1.0Ge1.0 were synthesized by direct reaction of elements
in quartz ampoule with alumina crucible. The initial high-purity 99.999%
germanium, 99.998% silicon, and 99.9% calcium were purchased from
Strem and Alfa Aesar, Germany. Tetrabutylammonium perchlorate was
obtained from Sigma-Aldrich, Czech Republic. Acetonitrile was obtained
from LachNer, Czech Republic. Acetic acid (p.a. purity) was purchased
from PENTA, Czech Republic. N-Methyl-2-pyrrolidone
(NMP) was delivered from Merck. Multiwalled carbon nanotubes were
obtained from BASF, and graphene oxide (GO) was made by the Tour method.
The electrolyte LiPF6 in the mixture of ethylene carbonate
(EC) and dimethyl carbonate (DMC) was purchased from Merck.
in quartz ampoule with alumina crucible. The initial high-purity 99.999%
germanium, 99.998% silicon, and 99.9% calcium were purchased from
Strem and Alfa Aesar, Germany. Tetrabutylammonium perchlorate was
obtained from Sigma-Aldrich, Czech Republic. Acetonitrile was obtained
from LachNer, Czech Republic. Acetic acid (p.a. purity) was purchased
from PENTA, Czech Republic. N-Methyl-2-pyrrolidone
(NMP) was delivered from Merck. Multiwalled carbon nanotubes were
obtained from BASF, and graphene oxide (GO) was made by the Tour method.
The electrolyte LiPF6 in the mixture of ethylene carbonate
(EC) and dimethyl carbonate (DMC) was purchased from Merck.
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