Mouse monoclonal anti c myc

Manufactured by Roche

The Mouse monoclonal anti-[c-myc] is a laboratory instrument used for the detection and analysis of the c-myc protein. It is an antibody that specifically binds to the c-myc protein, which is a transcription factor involved in cell growth and division. This product can be used in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the c-myc protein in biological samples.

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Lab products found in correlation

Nitrocellulose membrane, by Bio-Rad (2 mentions) Goat anti mouse igg hrp, by Bio-Rad (2 mentions) Amersham ecl plus, by GE Healthcare (1 mentions) Goat anti rabbit igg hrp, by Bio-Rad (1 mentions) Amersham ecl, by Cytiva (1 mentions) Rat anti ha, by Roche (1 mentions) Goat anti rat igg hrp, by Bio-Rad (1 mentions)

Close spelling variants of this product

Anti-Myc (45 citations) Anti-c-myc (15 citations) Mouse anti-Myc (15 citations) C-myc (10 citations) Mouse c-Myc (2 citations)

2 protocols using mouse monoclonal anti c myc

Western Blot Analysis of Tagged Proteins

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Yeast crude extracts were subjected to SDS-PAGE and proteins were transferred to nitrocellulose membrane (Bio-Rad). MORF-tagged and PT-tagged constructs were detected with rabbit polyclonal anti-protein A (Sigma, 1:5,000), followed by incubation with goat anti-rabbit IgG-HRP (Bio-Rad, 1:10,000), and visualization with Amersham ECL Plus (GE Healthcare). The 9myc tag was detected with mouse monoclonal anti-[c-myc] (Roche, 1:10,000), followed by incubation with goat anti-mouse IgG-HRP (Bio-Rad, 1:10,000), and visualization.

Guy M.P., Shaw M., Weiner C.L., Hobson L., Stark Z., Rose K., Kalscheuer V.M., Gecz J, & Phizicky E.M. (2015). Defects in tRNA anticodon loop 2'-O-methylation are implicated in non-syndromic X-linked intellectual disability due to mutations in FTSJ1. Human mutation, 36(12), 1176-1187.

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Affinity Purification of Yeast Proteins

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Yeast strains with C-terminal chromosomal tags (cMORF or myc) were grown in YPD to OD₆₀₀ 1–2.5 and crude extracts were made from 500–600 OD-mL pellets, followed by IgG Sepharose affinity purification and elution of bound protein with GST-3C protease. Then samples were subject to SDS-PAGE, transferred to nitrocellulose membrane (Bio-Rad), and probed with appropriate antibodies: mouse monoclonal anti-[c-myc] (Roche), followed by goat anti-mouse IgG-HRP (Bio-Rad); or rat anti-HA (Roche), followed by goat anti-rat IgG-HRP (Bio-Rad). Strains containing a P_GAL-ORF240-PT plasmid were grown as described in pull-down experiment.

Han L., Marcus E., D'Silva S, & Phizicky E.M. (2017). S. cerevisiae Trm140 has two recognition modes for 3-methylcytidine modification of the anticodon loop of tRNA substrates. RNA, 23(3), 406-419.

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