Cells were fixed in 4% paraformaldehyde (Sigma Aldrich, Gillingham, UK) prior to blocking at room temperature for 30 min with Phosphate Buffer Solution (PBS) (Lonza, Cologne, Germany) containing 10% normal donkey serum (Sigma‐Aldrich) 0.3% Triton X‐100 (Sigma‐Aldrich, Gillingham, UK) and 1% Bovine Serum Albumin (BSA) (Sigma‐Aldrich, Gillingham, UK). After blocking, samples were incubated with their respective conjugated antibodies in blocking buffer for 3 hours at room temperature in the dark. Conjugated antibodies used were Sox‐2 (NL557, R&D Systems), Oct‐3/4 (NL637, R&D Systems), and HAND1 (NL637, R&D Systems). Following a wash step in PBS, cells were incubated with 5 µM SYTO‐16 or DAPI (Life Technologies, Paisley, UK) solution for 10 min. A final wash in PBS was completed before viewing the samples in PBS on a Nikon Ti Eclipse microscope.
Phosphate buffer solution (pbs)
Manufactured by Lonza
Sourced in Germany, Switzerland
Phosphate Buffer Solution (PBS) is a commonly used buffer solution that maintains a stable pH. It is composed of a mixture of sodium phosphate and sodium chloride in water. PBS is widely used in various laboratory applications, including cell culture, immunoassays, and protein purification, where a stable and physiologically relevant pH is required.
Automatically generated - may contain errors
Lab products found in correlation
Normal donkey serum (nds), by Merck Group (1 mentions)
Nl557, by R&D Systems (1 mentions)
Nl637, by R&D Systems (1 mentions)
Eclipse ti microscope, by Nikon (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Glutaraldehyde solution, by Merck Group (1 mentions)
Mtt reagent, by Serva Electrophoresis (1 mentions)
Glacial acetic acid ch3cooh, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Serva Electrophoresis (1 mentions)
S aureus atcc 6538, by American Type Culture Collection (1 mentions)
E coli atcc 8739, by American Type Culture Collection (1 mentions)
2 protocols using phosphate buffer solution (pbs)
1
Immunofluorescence Staining for Stem Cell Markers
2
Chitosan-Alginate Hydrogel Scaffold Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (Cs) of low molecular
weight (89.9% degree of dealkylation) was purchased from Primex ehf,
Chitoclear, Iceland. Glacial acetic acid (CH3COOH) was
purchased from Thermo Fisher Scientific Inc. Sodium alginate (Protanal
LF 10/60 NF) was supplied by FMC BioPolymer, Philadelphia. Gelatin
was purchased from Honeywell Fluka Research Chemicals, Germany. MTT
reagent (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium
bromide) and dimethyl sulfoxide (DMSO) were purchased from Serva Electrophoresis,
Heidelberg, Germany. Phosphate buffer solution (PBS) was obtained
from Lonza, Switzerland. Fibrinogen type I-S (65–85% protein)
from bovine plasma, thrombin from bovine plasma (40–60% protein,
40–300 NIH units/mg protein), elastin from bovine neck ligament,
collagen type I solution from bovine skin, Dulbecco’s modified
Eagle’s medium (DMEM), poly(vinyl alcohol) (PVA) (Mw = 125 000 kDa), calcium sulfate dihydrate salt
(CaSO4. 2H2O), and glutaraldehyde solution (25%)
were purchased from Sigma Aldrich, Germany. E. coli (ATCC 8739) and S. aureus (ATCC 6538)
were purchased from the American Type Culture Collection.
weight (89.9% degree of dealkylation) was purchased from Primex ehf,
Chitoclear, Iceland. Glacial acetic acid (CH3COOH) was
purchased from Thermo Fisher Scientific Inc. Sodium alginate (Protanal
LF 10/60 NF) was supplied by FMC BioPolymer, Philadelphia. Gelatin
was purchased from Honeywell Fluka Research Chemicals, Germany. MTT
reagent (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium
bromide) and dimethyl sulfoxide (DMSO) were purchased from Serva Electrophoresis,
Heidelberg, Germany. Phosphate buffer solution (PBS) was obtained
from Lonza, Switzerland. Fibrinogen type I-S (65–85% protein)
from bovine plasma, thrombin from bovine plasma (40–60% protein,
40–300 NIH units/mg protein), elastin from bovine neck ligament,
collagen type I solution from bovine skin, Dulbecco’s modified
Eagle’s medium (DMEM), poly(vinyl alcohol) (PVA) (Mw = 125 000 kDa), calcium sulfate dihydrate salt
(CaSO4. 2H2O), and glutaraldehyde solution (25%)
were purchased from Sigma Aldrich, Germany. E. coli (ATCC 8739) and S. aureus (ATCC 6538)
were purchased from the American Type Culture Collection.
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