Dual luciferase reporter assay kit reagents

Manufactured by Promega

The Dual-Luciferase Reporter (DLR) Assay Kit reagents are a set of reagents designed to measure the activities of two luciferase reporter enzymes within a single sample. The kit includes firefly and Renilla luciferase assay reagents, which are used to measure the activity of these two reporter enzymes sequentially.

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Lab products found in correlation

X tremegene hp, by Roche (1 mentions) X tremegene hp dna transfection reagent, by Merck Group (1 mentions)

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Dual Luciferase Assay (854 citations) Dual luciferase kit (463 citations) Luciferase assay reagent (446 citations) Luciferase reporter assay (107 citations) Luciferase reagent (50 citations) Luciferase reporter kit (44 citations) Dual-luciferase reagent (29 citations) Dual-Luciferase Reporter reagent (10 citations) Luciferase reporter assay reagents (9 citations) Luciferase reporter reagent (4 citations)

2 protocols using dual luciferase reporter assay kit reagents

BMP4 and TGF-β1 Regulation of Luciferase Reporter

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BMP-luciferase (BRE-Luc) reporter plasmid [55 (link)] and SV-40 Renilla Luciferase (SV40-Luc) reporter plasmid were co-transfected into the C2C12 sublines using X-tremeGene HP as per the manufacturers recommendations (Roche). After 8 or 16 hours of transfection, DNA-lipid complexes were replaced with fresh medium -/+ BMP4 and/or -/+ TGF-β1. After an additional 8 or 20 hours, cell extracts were harvested for luminometer measurements using the Dual-luciferase reporter (DLR) assay kit reagents (Promega).

Alvarado E., Yousefelahiyeh M., Alvarado G., Shang R., Whitman T., Martinez A., Yu Y., Pham A., Bhandari A., Wang B, & Nissen R.M. (2016). Wdr68 Mediates Dorsal and Ventral Patterning Events for Craniofacial Development. PLoS ONE, 11(11), e0166984.

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Dual Luciferase Assay and Western Blot

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The pG5D1AISLAND-LUC (D1A) plasmid was previously described [41 (link)]. The luciferase and SV-40 Renilla plasmids were co-transfected into cells using X-tremeGENE HP DNA Transfection Reagent (6366244001, Sigma-Aldrich) per the manufacturer‘s recommendations. DNA-lipid complexes were replaced with fresh medium 16 hours later. After an additional 8 hours, cell extracts were harvested for luminometer measurements using the Dual-luciferase reporter (DLR) assay kit reagents (E1910, Promega). Four independent biological replicates of the transient transfection, each containing three technical replicates, were analyzed. Data were analyzed by one-way ANOVA and post-hoc Tukey HSD with p<0.05 as the threshold for significance.
For western blots, cells were transiently transfected with pEGFPC2, pEGFPC2-Wdr68 [25 (link)] or pEGFP-Dyrk1a [50 (link)] for 24hrs, medium refreshed for 24hrs, and then harvested for extracts.

Yousefelahiyeh M., Xu J., Alvarado E., Yu Y., Salven D, & Nissen R.M. (2018). DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B. PLoS ONE, 13(11), e0207779.

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