Glutaraldehyde

Manufactured by MP Biomedicals

Sourced in United States

Glutaraldehyde is a colorless, oily liquid chemical compound. It is a cross-linking agent that can be used in various laboratory and industrial applications.

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Lab products found in correlation

S 520, by Hitachi (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Triton x, by Promega (1 mentions) Jem 1230 tem, by JEOL (1 mentions) Em uc6i ultramicrotome, by Leica (1 mentions)

Spelling variants of this product

Glutaraldehyde (GA, (2 citations)

3 protocols using glutaraldehyde

SEM Analysis of MEF-ECM Morphology

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The morphology of MEF-ECM was observed by the
method previously described with minor modifications (15 (link)). The specimens were fixed with 2.5% glutaraldehyde
(MP Biomedicals, Irvine, CA, USA) for 30 minutes at
4˚C. After that, all samples were dehydrated with 60, 70,
80, 90 and 100% absolute ethanol for 20 minutes each
time and air dried. The dried samples were fixed on the
sample stage of the SEM using conductive adhesive and
subsequently sputter-coated in gold for 90 s. Imaging was
obtained by using a SEM (S-520; Hitachi, Japan) with a
voltage of 10-15 kV.

Zhang J., Liu L., Li Y., Wu J, & Lou X. (2023). Mouse Embryonic Fibroblasts-Derived Extracellular Matrix Facilitates Expansion of Inner Ear-Derived Cells. Cell Journal (Yakhteh), 25(7), 447-457.

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Visualizing LKB1 Localization in HeLa Cells

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HeLa cells (LKB1-null) were co-transfected with either pEGFP-C1 control or our panel of LKB1 constructs and Flag-STRADα using Lipofectamine 2000 (Invitrogen), per manufacturer’s protocol. 24 hours later, cells were fixed using Phemo buffer with a final concentration of: 3.7% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA), 0.05% glutaraldehyde (MP Biomedicals, Santa Ana, CA), and 0.5% Triton-X (Promega, Madison, WI) for 10 minutes at room temperature. Fixed cells were rinsed with PBS and stained with Alexa Fluor^® 555 Phalloidin (1:200 in PBS) for 1 hour, rinsed three times with PBS, and stained with 350 nM DAPI for 10 minutes followed by three more PBS washes. Coverslips were then mounted with ProLong^® Diamond Antifade Mountant. Fixed cells were imaged using a Leica SP8 inverted confocal microscope at 63x (HP PL APO 1.40 NA oil) using a 488 and 514 nm argon laser.

Wilkinson S., Hou Y., Zoine J.T., Saltz J., Zhang C., Chen Z., Cooper L.A, & Marcus A.I. (2017). Coordinated cell motility is regulated by a combination of LKB1 farnesylation and kinase activity. Scientific Reports, 7, 40929.

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Ultrastructural Analysis of Embryonic Epidermis

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Embryos were processed for EM at the VCU microscopy core using standard protocols. Briefly, embryos were fixed with 2% glutaraldehyde (MP Biomedicals, 198595) in 0.1M sodiμm cacodylate buffer (Electron Microscopy Services, 12300) overnight (4°C) and then refixed in 2% osmiμm tetroxide in 0.1M cacodylate buffer (one hour). They were dehydrated in ethanol and then infiltrated with propylene oxide (EMS, 20401) and Poly/Bed 812 resin mix (50:50, Polyscienes, 08792–1) overnight. Finally, embryos were incubated in EMbed 812 resin (EMS, 14120, overnight), placed in molds and heated 60°C (2 days). 700–900Å thick sections were created on a Leica EM UC6i Ultramicrotome (Leica Microsystems) and stained with 5% Uranyl acetate (EMS, 22400) and Reynold’s Lead Citrate (Lead Nitrate (EMS, 17900) and Sodium Citrate (EMS, 21140)). A JEOL JEM-1230 TEM (JEOL USA, Inc.) with the Gatan Orius SC1000 digital camera (Gatan Inc., Pleasanton, CA) was used to image the ultrastructure of the epidermis.

Bharathan N.K, & Dickinson A.J. (2019). Desmoplakin is required for epidermal integrity and morphogenesis in the Xenopus laevis embryo.. Developmental biology, 450(2), 115-131.

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