4 6 diamidino 2 phenylindole dapi c0060

Manufactured by Solarbio

Sourced in China

4',6-diamidino-2-phenylindole (DAPI, C0060) is a fluorescent dye commonly used in molecular biology and microscopy. It binds strongly to DNA, allowing for the visualization and quantification of nucleic acids.

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Lab products found in correlation

T8200, by Solarbio (1 mentions) Fluorescence microscope, by Leica camera (1 mentions) Triton x 100, by Merck Group (1 mentions) Ab215715, by Abcam (1 mentions) Ab58803, by Abcam (1 mentions) Ab179517, by Abcam (1 mentions) M1511 8, by Huabio (1 mentions) Sc 515599, by Santa Cruz Biotechnology (1 mentions) Anti tjp1, by Proteintech (1 mentions) Nmdar2b, by Abcam (1 mentions) Anti iba1, by Proteintech (1 mentions) Anti ocln, by Proteintech (1 mentions) Vglut1, by Abcam (1 mentions) Nmdar1, by Abcam (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Transstart tip green qpcr supermix, by Transgene (1 mentions) Transstart one step gdna removal and cdna synthesis supermix kit, by Transgene (1 mentions)

7 protocols using 4 6 diamidino 2 phenylindole dapi c0060

Immunofluorescence Staining of Frozen Tissue

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4 μm thick section slides from frozen human tissue were washed three times in PBS. Then the sections were permeabilized with 0.5% Triton X-100 (T8200, Solarbio) for 20 min. After 5% BSA incubation for 1 h, sections were incubated in ARPC1B and glial fibrillary acidic protein (GFAP) antibodies at 4°C overnight. Following incubation with fluorescein (FITC) or rhodamine (TRITC) secondary antibody and 4’,6-diamidino-2-phenylindole (DAPI, C0060, Solarbio), the samples were detected using fluorescence microscope (Leica). The images were merged digitally to monitor the co-localization condition.

Liu T., Zhu C., Chen X., Wu J., Guan G., Zou C., Shen S., Chen L., Cheng P., Cheng W, & Wu A. (2022). Dual role of ARPC1B in regulating the network between tumor-associated macrophages and tumor cells in glioblastoma. Oncoimmunology, 11(1), 2031499.

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Immunofluorescence Assay for PRV-XJ

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A total of 10 μL of rPRV-XJ-ΔTK/gE/gI-VP2 or PRV-XJ infected BHK-21 cells were cultured in DMEM containing 2% FBS in 6-well plates for 24 h. The cells were then fixed with immobile liquid (methanol and acetone, 1:1) at 4°C for 30 min, followed by blocking with 5% bovine serum albumin (BSA) at 20°C for 1 h. The cells were then incubated with primary antibody (1:200 dilution of mouse anti-VP2 polyclonal antibody) at 4°C for 16 h and with secondary antibody (1:500 dilution of Corallite 594-conjugated goat anti-mouse IgG [SA00013-3; Proteintech]) at 37°C for 1 h. 4′,6-Diamidino-2-phenylindole (DAPI; C0060; Solarbio) was used for nuclear staining.

Tao Q., Zhu L., Xu L., Yang Y., Zhang Y., Liu Z., Xu T., Wen J., Deng L., Zhou Y, & Xu Z. (2023). The Construction and Immunogenicity Analyses of a Recombinant Pseudorabies Virus with Senecavirus A VP2 Protein Coexpression. Microbiology Spectrum, 11(2), e05229-22.

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Immunofluorescence Staining of MDBK Cells

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MDBK cells were seeded in 24-well plates on glass coverslips and treated with 4% paraformaldehyde for 20 min at room temperature. After washing three times, cells were permeabilized with 1% (v/v) Triton X-100 (T8787, Sigma-Aldrich, St. Louis, MI, USA) for 15 min, and blocked in PBS with 2% (w/v) bovine serum albumin for 1 h. The cells were incubated with primary antibodies at 4 °C overnight. Then, the cells were incubated with Alexa Fluor 488 goat anti-rabbit secondary antibodies (Beyotime Biotechnology, Shanghai, China) for 1 h at room temperature. 4′-6-diamidino-2-phenylindole (DAPI, C0060, Solarbio Science & Technology Co., Ltd., Beijing, China) was used to stain nuclei. Images were acquired by confocal microscopy.

Zhang L., Yang G., Wang J., Zhang J., Chen K., Xiong X., Zhu Y., Xu C, & Wang J. (2023). Ethyl Gallate Inhibits Bovine Viral Diarrhea Virus by Promoting IFITM3 Expression, Lysosomal Acidification and Protease Activity. International Journal of Molecular Sciences, 24(10), 8637.

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Comprehensive Immunohistochemical Characterization

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Briefly, the sections had been repaired with the antigen repair solution, observed by using 0.5% Triton X-100 permeabilized, and blocked with 5% (w/v) bovine serum albumin (BSA). Then the sections were incubated with primary antibodies at 4°C overnight. Primary antibodies used were as follows: TGF-β1 (ab215715, Abcam, 1:200), MMP9 (ab58803, Abcam, 1:200), Platelet endothelial cell adhesion molecule-1 (CD31) (M1511-8, Huabio, 1:200), Cleaved caspase-3 (ab179517, Abcam, 1:200), NKG2-D (sc-515599, Santa Cruz, 1:200), and nucleotide-binding oligomerization domain containing 2 (NOD2) (sc-56168, Santa, 1:200). The slides were incubated with secondary antibodies for 60 min. Finally, nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (c0060, Solarbio), and the slides were visualized by fluorescence microscopy.

Cao G.Z., Tian L.L., Hou J.Y., Zhang Y., Xu H., Yang H.J, & Zhang J.J. (2024). Integrating RNA-sequencing and network analysis to explore the mechanism of topical Pien Tze Huang treatment on diabetic wounds. Frontiers in Pharmacology, 14, 1288406.

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Immunofluorescence Antibody Staining Protocol

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Anti-IBA1, anti-TJP1, and anti-OCLN were purchased from ProteinTech, Wuhan, China. Anti-P-synapsin-1, VGlut1, NMDAR1, and NMDAR2B were purchased from Abcam, Shanghai, China. 4,6-Diamidino-2-phenylindole (DAPI; #C0060) was purchased from Solarbio, Beijing, China.

Gu X., Bi N., Wang T., Huang C., Wang R., Xu Y, & Wang H.L. (2022). Probiotic Lactobacillus rhamnosus GR-1 supplementation attenuates Pb-induced learning and memory deficits by reshaping the gut microbiota. Frontiers in Nutrition, 9, 934118.

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Epigenetic Histone Modification Analysis

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Anti-H3K27me3 (#ab6002), anti-H3K4me3 (#ab8580), anti-H3K4me2 (#ab32356), and anti-H3 antibodies (#ab1791) were purchased from Abcam China (Shanghai, China). Anti-EZH2 (#07-689) antibody was purchased from EMD Millipore (Shanghai, China). Anti-β-actin (#4970S) was purchased from Cell Signaling Technology (Shanghai, China). DAPI (4,6-diamidino-2-phenylindole; #C0060) was purchased from Solarbio (Beijing, China). All other reagents were of the highest analytical grade.

Xiao J., Wang T., Xu Y., Gu X., Li D., Niu K., Wang T., Zhao J., Zhou R, & Wang H.L. (2020). Long-term probiotic intervention mitigates memory dysfunction through a novel H3K27me3-based mechanism in lead-exposed rats. Translational Psychiatry, 10, 25.

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Transcriptome Analysis of Cholesterol Metabolism

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The TransStart one-step gDNA removal and cDNA synthesis supermix kit (AT311-02) and TransStart Tip Green qPCR supermix (AQ142-21) were ordered from TransGen Biotech (Beijing, China), The total RNA kit was ordered from Omega (Guangzhou, China), BODIPY 493/503 (GC42959) was purchased from GlpBio (Montclair, CA, USA), DAPI (4′,6-diamidino-2-phenylindole) (C0060) was ordered from Solarbio Science & Technology (Beijing, China), and 25-hydroxycholesterol (25HC) (HY-113134) and asiaticoside (AS) (HY-N0439) were ordered from MedChemExpress (Monmouth Junction, NJ, USA).

Zhang J., Yang G., Wang X., Zhu Y, & Wang J. (2022). 25-Hydroxycholesterol Mediates Cholesterol Metabolism to Restrict Porcine Deltacoronavirus Infection via Suppression of Transforming Growth Factor β1. Microbiology Spectrum, 10(6), e02198-22.

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