Syk inhibitor

Manufactured by Merck Group

The SYK inhibitor is a laboratory reagent used in research applications. It functions by inhibiting the activity of the spleen tyrosine kinase (SYK) enzyme, which plays a role in various cellular processes. The product is intended for use in in vitro research settings.

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Syk inhibitor (piceatannol) (2 citations)

2 protocols using syk inhibitor

Investigating SYK and NF-κB Signaling

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Unless stated otherwise, all chemicals were purchased from MilliporeSigma. Antibodies were obtained from the following sources: anti–p-Tyr (catalog 9411), anti–p-SRC (Y416) (catalog 2101), anti-SRC (catalog 2109), anti–p-SYK (Y525/526) (catalog 2710), anti-SYK (catalog 2712), anti–p-IκBα (catalog 2859), anti-IκBα (catalog 9242), anti-GAPDH (catalog 2118), anti-TLR2 (catalog 12276 for human and 13744 for mouse), anti–NF-κB (catalog 3033), and anti–NF-κB (catalog 8242) from Cell Signaling Technology; anti-MyD88 (catalog SAB3500472) and anti-FLAG M2 (catalog F3165) from MilliporeSigma; anti-Myc (catalog sc-40) and anti-actin (catalog sc-47778) from Santa Cruz Biotechnology; anti-HA (M180-3) from MBL International; anti–p-Tyr (4G10) (catalog 05-321) from Millipore; anti-PPP1R11 (ab171960) from Abcam; and anti-3BP2 (catalog H00006452-M01) from Abnova. Halt Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scientific. SYK inhibitor was from Millipore. PP2 and BMS 345541 were from Selleckchem.

Matsumoto Y., Dimitriou I.D., La Rose J., Lim M., Camilleri S., Law N., Adissu H.A., Tong J., Moran M.F., Chruscinski A., He F., Asano Y., Katsuyama T., Sada K.E., Wada J, & Rottapel R. (2022). Tankyrase represses autoinflammation through the attenuation of TLR2 signaling. The Journal of Clinical Investigation, 132(7), e140869.

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Inflammatory Mediators and Inhibitors

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The following reagents were used in this study: Lipopolysaccharide (ultrapure LPS), N-Acetyl-L-cysteine (NAC – A7250, Sigma Aldrich), Carbenoxolone (CBX – C4790, Sigma Aldrich), Nigericin (Nig – N7143, Sigma Aldrich). The Kappa-Carrageenan (Cg) was obtained from BDH Biochemicals (Poole, England Cat. n° 38,100). The concentration used for the in vitro experiments range from 30 μg/ml up to 300 μg/ml. KCl (Cat. n°. 7447–40-7, Labsynth), Caspase-1 inhibitor (Calbiochem, Cat. n°. 400,010), Syk inhibitor (Millipore, Cat. n°. 574,711).

Lopes A.H., Silva R.L., Fonseca M.D., Gomes F.I., Maganin A.G., Ribeiro L.S., Marques L.M., Cunha F.Q., Alves-Filho J.C., Zamboni D.S., Lopes N.P., Franklin B.S., Gombault A., Ramalho F.S., Quesniaux V.F., Couillin I., Ryffel B, & Cunha T.M. (2020). Molecular basis of carrageenan-induced cytokines production in macrophages. Cell Communication and Signaling : CCS, 18, 141.

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