C57bl6 nj wt

Manufactured by Jackson ImmunoResearch

Sourced in United States

C57BL6/NJ WT is a laboratory mouse strain commonly used in biomedical research. It is a wild-type (WT) strain, meaning it has the natural, unmodified genome. This strain is widely utilized as a control or reference strain in various experimental studies.

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Lab products found in correlation

Caspase 1 11, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Pentobarbital sodium, by Henry Schein (1 mentions)

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WT) C57BL6/NJ mice (3 citations)

3 protocols using c57bl6 nj wt

Neutrophil Influx upon ATP Induced Inflammation

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Animal experimentation protocol (004–15) was approved by the Animal Ethics Committee from Institut Pasteur de Montevideo. All methods were performed in accordance with the relevant guidelines and regulations. 6–10 weeks old C57BL6/NJ WT and caspase 1–11^−/− from Jackson Laboratories were used. 20 mg/kg ATP was injected i/p to saline or HCQ (1 mg/kg) i/p treated animals. HCQ was done daily since day - 7. 4 hs after ATP injection, peritoneal lavage was done. Neutrophils were quantified by FACS using anti-CD11b and anti-Ly6G antibodies.

Eugenia Schroeder M., Russo S., Costa C., Hori J., Tiscornia I., Bollati-Fogolín M., Zamboni D.S., Ferreira G., Cairoli E, & Hill M. (2017). Pro-inflammatory Ca++-activated K+ channels are inhibited by hydroxychloroquine. Scientific Reports, 7, 1892.

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Genetic Deletion of GSDMD and Caspases

Cited 1 time

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Gsdmd^−/− mice (in a C57BL/6 J background) were generated as previously described^{53 (link)}. Casp1/11^-/- (in a C57BL/6NJ background), C57BL/6 J WT, and C57BL/6NJ WT mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Eight- to 12-week-old mice were used in all experiments. Corresponding sex- and age-matched WT mice were used as controls in all experiments performed with KO mice. The Boston Children’s Hospital Animal Care and Use Committee approved and monitored all mouse procedures.

Ding X., Kambara H., Guo R., Kanneganti A., Acosta-Zaldívar M., Li J., Liu F., Bei T., Qi W., Xie X., Han W., Liu N., Zhang C., Zhang X., Yu H., Zhao L., Ma F., Köhler J.R, & Luo H.R. (2021). Inflammasome-mediated GSDMD activation facilitates escape of Candida albicans from macrophages. Nature Communications, 12, 6699.

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Kv1.1 KO Mice Cardiac Tissue Isolation

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The Kvβ1.1 KO (global; Giese et al. 1998 (link)) and C57BL6/NJ (WT) female and male mice were obtained from Jackson Laboratories (stock number 007747; Bar Harbor, ME, USA). Mice were used at 12–14 weeks of age (20 female mice with average weights of 19–21 g and 15 male mice with average weights of 26–28 g, of each strain). All animal protocols and use were approved by the Institutional Animal Care and Use Committee at the University of South Florida (Tampa, FL, USA), which is consistent with the practices approved by US National Institutes of Health guidelines. The investigators understand the ethical principles under which the journal operates and that this work complies with the journal’s animal ethics checklist. All the mice had continuous access to food and water, ad libitum. Mice were injected with heparin (360 USP; Sigma) and killed with Somnasol [Pentobarbital Sodium, 50 mg (kg body weight)⁻¹, Henry Schein Animal Health, Dublin, OH, USA] by I.P. injection, and heart tissue was excised after thoracotomy, snap frozen in liquid N₂ and stored at −80°C until further use.

Tur J., Chapalamadugu K.C., Padawer T., Badole S.L., Kilfoil PJ I.I., Bhatnagar A, & Tipparaju S.M. (2016). Deletion of Kvβ1.1 subunit leads to electrical and haemodynamic changes causing cardiac hypertrophy in female murine hearts. Experimental physiology, 101(4), 494-508.

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