Hydrogen peroxide

Manufactured by Roche

Hydrogen peroxide is a chemical compound with the formula H2O2. It is a clear, colorless liquid that is commonly used as an oxidizing agent, disinfectant, and bleaching agent in various applications.

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Lab products found in correlation

Iview dab detection kit, by Roche (1 mentions) Cc1 buffer, by Roche (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Formalin, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Oil red o stain kit, by StatLab (1 mentions) Bluing reagent, by Roche (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Hematoxylin 2 counterstain reagent, by Roche (1 mentions)

Spelling variants of this product

Amplex Red Hydrogen Peroxide/Peroxidase Assay (2 citations)

2 protocols using hydrogen peroxide

Immunohistochemical Pepsin Detection in Laryngeal Tissue

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Biopsy specimens of the laryngeal tissue were obtained during microlaryngoscopy procedures. Paraffin-embedded sections (2–3-μm thick) were prepared from the biopsy samples (Ventana Medical Systems, AZ, USA) and analyzed at the Department of Pathology by a single pathologist. Immunohistochemical analysis was performed after endogenous peroxidase blocking with hydrogen peroxide (Ventana) and antigen revitalization in CC1 buffer (Ventana). A pepsin antibody (NB100-66518, Novus Biologicals, CO, USA, diluted at a ratio of 1:100) was used as the primary antibody to detect pepsin. The incubation period for the primary antibody was 32 min. The iView DAB Detection Kit (Roche, Switzerland) was used to visualize the antigens. The presence of any antibody positivity in the cytoplasm of the cells was considered to be pathological and the sample was evaluated as pepsin positive.

Formánek M., Jančatová D., Komínek P., Tomanová R, & Zeleník K. (2017). Comparison of Impedance and Pepsin Detection in the Laryngeal Mucosa to Determine Impedance Values that Indicate Pathological Laryngopharyngeal Reflux. Clinical and Translational Gastroenterology, 8(10), e123-.

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Immunohistochemistry and Oil Red O Staining

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Acetonitrile, ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), formalin, formic acid, phenylmethylsulfonyl fluoride (PMSF), phosphate-buffered saline (PBS), paraformaldehyde (PFA) and protease inhibitor cocktail were purchased from Sigma-Aldrich (St. Louis, MO). Reaction buffer (10X), Discovery CC1, Discovery PSS diluent, Discovery OmniMap anti-Rb HRP (RUO), OmniMap DAB anti-Rb detection kit, Hematoxylin II counterstain reagent, hydrogen peroxide, and Bluing reagent were purchased from Ventana Medical Systems (Tucson, AZ). Oil Red O stain kit was sourced from American MasterTech (Lodi, CA).

Pan Y., Omori K., Ali I., Tachikawa M., Terasaki T., Brouwer K.L, & Nicolazzo J.A. (2018). Altered expression of small intestinal drug transporters and hepatic metabolic enzymes in a mouse model of familial Alzheimer’s disease. Molecular pharmaceutics, 15(9), 4073-4083.

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