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4 protocols using tetrachloroauric acid trihydrate haucl4 3h2o

1

Synthesis and Characterization of Gold Nanoparticles

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Tetrachloroauric acid trihydrate (HAuCl4·3H2O, 99.9%), dopamine hydrochloride (98%), NaNO2 (97%), H2SO4 (95%), HCl (37%), NaCl (99%), KCl (99%), NaH2PO4 (99%), K2HPO4 (99%), K4Fe(CN)6·3H2O (99%) and K3Fe(CN)6 (99%) were purchased from Sigma-Aldrich (Germany) and used as received. Phosphate-buffered saline solutions (PBS, 10 mM) were prepared by dissolving NaCl, KCl, Na2HPO4 and KH2PO4 in deionized water (>18.2 MΩ cm).
All stock solutions were prepared by dissolving the desired amount of the chemical in PBS 10 mM solution unless otherwise specified.
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2

Synthesis of Gold Nanoparticles and Conjugates

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Tetrachloroauric acid trihydrate (HAuCl4 · 3H2O) was purchased from Sigma-Aldrich (Shanghai, China). Silver nitrate (AgNO3), ascorbic acid (AA), trisodium citrate, hydrochloric acid (HCl), fluorouracil (5-FU), cisplatin (CisPt), and camptothecin (CAMP) were purchased from Aladdin Chemistry Co. Ltd (Shanghai, China). Custom-made peptides such as nuclear localization signal (NLS, CGGGPKKKRKVGG) and cell penetrating peptide (RGD, RGDRGDRGDRGDPGC) were procured from Sangon Biotech (Shanghai, China). Thiol-modified methoxypolyethylene glycol (mPEG-SH, MW 5000) was obtained from Jenkem Technology Co. Ltd (Beijing, China).
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3

Spectroscopic DNA Characterization Protocols

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Zinc
nitrate hexahydrate (Zn(NO3)2·6H2O) was bought from Merck Ltd. All
DNAs; the synthetic polynucleotides (poly(dA)·poly(dT) and poly(dG)·poly(dC));
the corresponding monophosphate residues of the DNA; and the purine
and pyrimidine base components, i.e., adenine hydrochloride hydrate,
thymine, tetrachloroauric acid trihydrate (HAuCl4·3H2O), and Hoechst 33258, were obtained from Sigma-Aldrich. The
reduced form of l-glutathione (GSH) was purchased from Alfa
Aesar. All of the compounds were treated without any further purification.
Ultrapure water (18.2 MΩ cm) was used throughout the experiment.
All of the DNA solutions were prepared as previously reported.9 (link) The concentrations of calf thymus (CT), E. coli (EC), and M. lysodeikticus (ML) DNAs were determined spectroscopically from the absorbance
value using their corresponding molar absorption coefficients.9 (link)
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4

Synthesis of Gold Nanoparticles with Vancomycin

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All of the materials and reagents were analytical quality. Vancomycin (purity ≥85%, CAS no. 1404-93-9) Polyethyleneimine (50% w/v in H2O; CAS no. 9002-98-6), tetra-chloroauric acid trihydrate (HAuCl4.3H2O; purity 99.9%, CAS no. 16961-25-4), and formaldehyde (≥36.0% in H2O) were purchased from Sigma Aldrich (Mumbai, India). Mercuric chloride (purity; ≥99.5%), potassium chloride (purity; 99%), sodium chloride (99%), magnesium chloride, and ammonium chloride were purchased from Merck (Bangalore, India). Other required glassware and plastic wares were purchased from Tarson (Mumbai, India). All of the experiments were performed using ultra-purified HPLC-grade water.
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