The mice were fed with HFD (MP Biomedicals) containing 60 kcal% calorie value, which was replaced every three days and weighted to calculate food intake. When necessary, mice were subcutaneously injected with either vehicle or LPZ (10 mg/kg) daily until the completion of the experiments, when serum, different adipose depots and liver samples were dissected and weighted for subsequent analysis. The bone and fat volume of mice was recorded using the SKyScan micro-CT scanner (Bruker). Volumetric micro-CT data were post-processed via Gaussian smoothing, and analyzed using the CTAn software (Bruker), wherein regions of interest were manually delineated. Energy expenditure was measured by the Promethion calorimetry system (Sable Systems International).
Promethion calorimetry system
Manufactured by Sable Systems
Sourced in United States
The Promethion calorimetry system is a laboratory equipment designed to measure the metabolic rate and energy expenditure of small animals or other biological samples. It provides precise and reliable data on oxygen consumption, carbon dioxide production, and respiratory exchange ratio.
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Lab products found in correlation
2 protocols using promethion calorimetry system
1
High-Fat Diet Induced Obesity Protocol
2
Measuring Energy Expenditure in Mice
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Eight-week-old chow-fed male mice were housed in either cold (10 °C) or thermoneutrality (30 °C) for 4 weeks. Animals were anaesthetised using 90 mg/kg of pentobarbital by intraperitoneal injection and were placed in 2.7 l calorimetry chambers (Oxymax, Columbus instruments, Ohio) attached to a Promethion calorimetry system (Sable Systems, Las Vegas, NV, USA) pre-warmed to 30 °C for 20 min for measurement of basal energy expenditure. They were then given a subcutaneous injection of 2 µl/g of 0.5 mg/mL noradrenaline bitartrate (NA) plus 1.66 µl/g of 18 µg/µl pentobarbital and NA-stimulated energy expenditure was measured for 25 min. Animals were then sacrificed, and tissues snap frozen as described above. Basal energy expenditure was calculated from the three readings prior to NA-injection. Peak energy expenditure was calculated from the three greatest readings 5–25 min after NA-injection. NA-induced energy expenditure was calculated as the difference between peak and basal energy expenditure.
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