Penicillin, streptomycin and DAPI (4', 6-diamidino-2-phenylindole) were purchased from Sigma-Aldrich (St. Louis, MO, USA). OPG, M-CSF and RANKL were obtained from PeproTech Inc. (Rocky Hill, CT, USA). Dulbecco’s modified Eagle’s medium (DMEM), α-MEM and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Trypsin was obtained from Amresco (Solon, OH, USA). Antibodies against Bax, Bcl-2, cleaved-caspase-3, cleaved-caspase-9, cleaved-caspase-8, cytochrome c and β-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Antibodies against Fas and Fas Ligand were purchased from Boster (Pleasanton, CA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin G (IgG) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other chemicals and reagents were purchased locally at analytical grade.
Antibodies against bax
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against Bax are laboratory reagents used to detect the presence and distribution of the Bax protein in various biological samples. Bax is a pro-apoptotic member of the Bcl-2 protein family and plays a key role in the regulation of programmed cell death (apoptosis).
Automatically generated - may contain errors
Lab products found in correlation
Bcl 2, by Cell Signaling Technology (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
β actin, by Cell Signaling Technology (3 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
Rankl, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Avantor (1 mentions)
Cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated goat anti rabbit immunoglobulin g igg, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 8, by Cell Signaling Technology (1 mentions)
P jnk, by ABclonal (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
P perk, by ABclonal (1 mentions)
P ire1, by ABclonal (1 mentions)
6 protocols using antibodies against bax
1
Investigating Apoptosis Pathways in Osteoclast Differentiation
2
Investigating ER Stress Pathways in PEDV Infection
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The ER stress inhibitor 4-phenylbutyrate (4-PBA) and a pancaspase inhibitor were obtained from MCE (Shanghai, China). All inhibitors were solubilized in dimethyl sulfoxide (DMSO; Solarbio, Beijing, China). Inhibitors were diluted, added to culture medium, and cultivated for an hour before infection. Antibodies against Bax, Bcl-2, caspase-3, caspase-9 and CHOP were purchased from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against GRP78, PERK, p-PERK, eIF2, p-eIF2, IRE1, p-IRE1, JNK, p-JNK and β-actin were purchased from ABclonal (Wuhan, China). An antibody against IRX1 was purchased from ABmart (Shanghai, China), and antibodies against Flag-tagged, GFP-tagged and PE-conjugated anti-mouse IgG were purchased from TRAN (Beijing, China). A polyclonal antibody directed against the PEDV (CH/SXYL/2016) N protein and PEDV Nsp9 protein was prepared in our laboratory.
3
Auraptene and NAC Modulate Oxidative Stress and Apoptosis in Glioblastoma
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Auraptene and N-acetyl-cysteine (NAC) were purchased from Cayman Chemical (Michigan, MI, USA). The human malignant GBM (U87) cell line was obtained from the National Cell Bank of Iran (NCBI), Pasteur Institute (Tehran, Iran). The Dichloro-dihydro-fluorescein diacetate (DCFDA)/ H2DCFDA- cellular ROS detection assay kit was obtained from Abcam (Cambridge, United Kingdom). Annexin V-FITC early apoptosis assay kit and antibodies against Bax, Bcl-2, and β-actin were prepared from Cell Signaling Technology (Beverly, MA, USA). Dimethyl sulfoxide (DMSO), gelatin, trypsin-EDTA, and penicillin-streptomycin were provided from Sigma-Aldrich (St. Louis, MO, USA). The bicinchoninic acid (BCA), protein assay kit, was produced by Pierce Co. (Pierce, Rockford, IL, USA). Fetal bovine serum (FBS) and High Glucose-Dulbecco's Modified Eagle's medium (DMEM) were obtained from Gibco (Grand Island, NY, USA).
4
Synthesis and Evaluation of Near-Infrared Dye
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Indocyanine green (ICG), human serum albumin (HSA, ≥98%), bovine serum albumin (BSA, ≥98%) were purchased from Macklin Inc. (Shanghai, China), Dulbecco’s modified Eagle’s medium and fetal bovine serum (FBS) were purchased from BasalMedia (Shanghai, China), trypsin-EDTA, penicillin-streptomycin were supplied by Solarbio (Shanghai, China). 3-(4,5-diMethylthialzol-2-yl)-2,5-diphe-nyltetrazolium bromide (MTT) and Hoechst33342 were purchased from YuanYe Bio-Technology (Shanghai, China). The universal SP kit (mouse/rabbit streptavidin–biotin detection system), DAB kit and PBS buffer (pH 7.4) were purchased from Zsbio (Beijing, China). All-purpose Powerful Antigen Retrieval Solution and Calcein/PI Live/Dead Assay kit and antibodies against Bcl-2 were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Protein Loading Buffer, Multicolor Prestained Protein Ladder and 10% SDS-PAGE were from EpiZyme (Shanghai, China). Near-infrared dye IR-817 was synthesized following our previously reported protocol.26 (link) Antibodies against Ki-67 was purchased from Cell Signaling Technology (CST, Beverly, MA, USA). Antibodies against Cleaved caspase-3 (CC-3) was purchased from Zen-bio (Chengdu, China). Antibodies against Bax was purchased from Cell Signaling Technology (CST, Beverly, MA, USA).
5
Molecular Pathway Modulation Protocol
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The following chemicals were used at doses indicated in the text and figures. Etoposide and the FDA-approved Drug Library were purchased from Meryer (Shanghai, China) and Selleck (Houston, TX, USA), respectively. Antibodies against CYP2C19, XPC, and PPARα were obtained from Abgent (San Diego, CA, USA). Antibodies against BAX, BAK, NFBKIβ, EGFR, and FOS were purchased from Cell Signaling Technology (Danvers, MA, USA). NAT2 antibody was purchased from OmnimAbs (Alhambra, CA, USA). TPMT antibody was obtained from 4A Biotech (Beijing, China). UGCG antibody was purchased from Proteintech (Chicago, IL, USA). β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). siRNAs targeting EGFR, FOS, NFKB1, PPARα, RXRα, RXRβ, TPMT, UGCG, and XPC were obtained from Qiagen (Hilden, Germany). All reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.
6
Cytotoxicity and Apoptosis Assays
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Trypsin, chloramphenicol, acridine orange, quercetin (QE), beta-carotene, (+)-catechin, butylated hydroxytoulene (BHT), proteinase K, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-(4,5-dimethylthylthiazol-2yl)-2,5-diphynyl tetrazolium bromide (MTT), Nonidet P-40, RNAse A and ethidium bromide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nonessential amino acid, sodium biocarbonate, Dulbecco'smodi ed Eagle's medium (DMEM), L-glutamine and gallic acid were purchased from HiMedia (India). StepupTM 1kb DNA ladder was purchased from Merck Biosciences, Bangalore (India). Fetal Bovine Serum (FBS) and Trypsin-EDTA were purchased from GIBCO-BRL. MDA-MB-231 (human breast cancer cells) and HEK 293 T (non-cancerous cell line) were procured from the National Center for Cell Science (NCCS), Pune, India. Antibodies against Bax, Bcl-2, cleaved PARP-1 and β-actin were purchased from Cell Signaling Technology (Bedford, Massachusetts, USA). All other reagents and media components used were of analytical grade and were purchased from Sigma chemicals, Himedia, Merck or other local commercial sources unless otherwise mentioned.
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